A rapid derivatization based LC–MS/MS method for quantitation of short chain fatty acids in human plasma and urine

• Published in: Bioanalysis Vol. 11; no. 8; pp. 741 - 753
• Main Authors: Jaochico, Allan, Sangaraju, Dewakar, Shahidi-Latham, Sheerin K
• Format: Journal Article
• Language: English
• Published: England Future Science Ltd 01.04.2019; Newlands Press
• Subjects: 2-methylbutyric acid; 3-methylvaleric acid; biomarker; butyric acid; Calibration; caproic acid; Charcoal; derivatization; Disease; Fatty acids; Feces; Homeostasis; human plasma and urine; Inflammatory bowel disease; Inflammatory bowel diseases; Intestine; Ionization; Irritable bowel syndrome; isobutyric acid; isocaproic acid; isovaleric acid; Ligands; liquid–liquid extraction; Metabolism; Methods; Microbiota; Plasma; propionic acid; Quality control; Quality standards; Quantitation; Reagents; reversed-phase LC–MS/MS; short-chain fatty acid; Urine; valeric acid; United States > US; 2-methylbutyric acid; reversed-phase LC–MS/MS; isobutyric acid; derivatization; biomarker; 3-methylvaleric acid; isocaproic acid; propionic acid; short chain fatty acid; valeric acid; isovaleric acid; human plasma and urine; butyric acid; caproic acid; liquid–liquid extraction
• ISSN: 1757-6180; 1757-6199
• DOI: 10.4155/bio-2018-0241

Objective of this study is to develop a robust multi-matrix LC–MS/MS for the quantitation of endogenous short-chain fatty acids (SCFA) biomarkers in human plasma and urine. Developed method utilizes stable isotope-labeled internal standards, high-throughput derivatization procedure for sample preparation and LC–MS/MS analysis using multiple reaction monitoring transitions in positive electrospray ionization mode. Surrogate matrix method was used for quantitation. Accuracy, precision, parallelism, curve linearity, derivatization efficiency, stability and recovery were all evaluated, and the results were well within the acceptable criteria. SCFA levels in human plasma and urine of inflammatory bowel disease patients versus non-disease subjects were quantified and compared by LC–MS/MS.