Melanoidin-containing wastewaters induce selective laccase gene expression in the white-rot fungus Trametes sp. I-62

• Published in: Research in microbiology Vol. 159; no. 2; pp. 103 - 109
• Main Authors: González, Tania, Terrón, María Carmen, Yagüe, Susana, Junca, Howard, Carbajo, José María, Zapico, Ernesto Javier, Silva, Ricardo, Arana-Cuenca, Ainhoa, Téllez, Alejandro, González, Aldo Enrique
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.03.2008
• Subjects: Basidiomycete; Biodegradation; Biodegradation, Environmental; Decolorization; Fungi; Gene Expression Regulation, Fungal; Laccase; Laccase - genetics; Laccase - metabolism; Ligninolytic enzymes; Melanoidin; Molasses - analysis; Molasses wastewaters; Polymers - metabolism; Polyporales - enzymology; Polyporales - genetics; Polyporales - metabolism; Trametes; Transcription, Genetic; Waste Disposal, Fluid; Molasses wastewaters; Fungi; Basidiomycete; Decolorization; Ligninolytic enzymes; Melanoidin; Laccase
• ISSN: 0923-2508; 1769-7123
• DOI: 10.1016/j.resmic.2007.10.005

Wastewaters generated from the production of ethanol from sugar cane molasses may have detrimental effects on the environment due to their high chemical oxygen demand and dark brown color. The color is mainly associated with the presence of melanoidins, which are highly recalcitrant to biodegradation. We report here the induction of laccases by molasses wastewaters and molasses melanoidins in the basidiomycetous fungus Trametes sp. I-62. The time course of effluent decolorization and laccase activity in the culture supernatant of the fungus were correlated. The expression of laccase genes lcc1 and lcc2 increased as a result of the addition of complete molasses wastewater and its high molecular weight fraction to fungal cultures. This is the first time differential laccase gene expression has been reported to occur upon exposure of fungal cultures to molasses wastewaters and their melanoidins.