Exposure to 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin and tetraethyl lead affects lung mitochondria bioenergetics

• Published in: Toxicology mechanisms and methods Vol. 20; no. 1; pp. 1 - 6
• Main Authors: Simões, Anabela Marques, Duarte, Filipe Valente, Teodoro, João Soeiro, Rolo, Anabela Pinto, Palmeira, Carlos Marques
• Format: Journal Article
• Language: English
• Published: England Informa UK Ltd 01.01.2010; Taylor & Francis
• Subjects: Adenosine Triphosphatases - metabolism; Adenosinetriphosphatase; Animals; Bioenergetics; Data processing; Dioxin; Dioxins; Energy Metabolism - drug effects; Environmental pollutants; Environmental Pollutants - toxicity; In Vitro Techniques; Lead; Lung; Lung - drug effects; Lung - metabolism; Lung diseases; Membrane Potential, Mitochondrial - drug effects; Membrane Potential, Mitochondrial - physiology; Mitochondria; Mitochondria - drug effects; Mitochondria - metabolism; Mitochondria - physiology; Mitochondrial Membrane Transport Proteins - drug effects; Mitochondrial Membrane Transport Proteins - physiology; Mitochondrial Swelling - drug effects; Mitochondrial Swelling - physiology; Oxidative phosphorylation; Oxidative Phosphorylation - drug effects; Oxygen Consumption - drug effects; Pollutants; Polychlorinated Dibenzodioxins - toxicity; Respiration; Swine; TCDD; Tetraethyl Lead - toxicity; Toxicity
• ISSN: 1537-6516; 1537-6524
• DOI: 10.3109/15376510903527916

Environmental pollutants such as TCDD and tetraethyl lead are extremely toxic and related with pulmonary disease development. Lung mitochondria are primary cellular targets for dioxins exposure-induced toxicity. TCDD showed a delay in the repolarization after a phosphorylative cycle and a decrease on state 3 respiration, suggesting alterations at the phosphorylative system level. The ATPase activity showed no differences between control and lung mitochondria incubated with TCDD, implying alterations in other components of the phosphorylative system. Tetraethyl lead also showed a delay in the repolarization after a phosphorylative cycle and a decrease on RCR. These data suggest that lung mitochondria incubated with TCDD and tetraethyl lead showed impaired mitochondrial function, reflecting the loss of oxidative phosphorylation capacity.