A new genus of the order Actinomycetales, Virgosporangium gen. nov., with descriptions of Virgosporangium ochraceum sp. nov. and Virgosporangium aurantiacum sp. nov

• Published in: International journal of systematic and evolutionary microbiology Vol. 51; no. 5; pp. 1809 - 1816
• Main Authors: Tamura, T, Hayakawa, M, Hatano, K
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.09.2001; Society for General Microbiology
• Subjects: Acinomycetales; Bacteriology; Biological and medical sciences; DNA, Ribosomal - analysis; Fatty Acids - analysis; Fundamental and applied biological sciences. Psychology; Microbiology; Micromonosporaceae - chemistry; Micromonosporaceae - classification; Micromonosporaceae - genetics; Micromonosporaceae - growth & development; Micromonosporaceae - ultrastructure; Molecular Sequence Data; Mycology; Nucleic Acid Hybridization; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Spores, Bacterial - ultrastructure; Systematics; Virgosporangium aurantiacum; Virgosporangium ochraceum; Asia; Japan; Glutamic acid; Alanine; New genus; Description; Sample; Sporangiophore; Spores; Strain; Actinomycetales; Substrate; Soils; Aminoacid; Neurotransmitter; Bacteria; Isolate; Isolation; Actinomycetes; Culture
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/00207713-51-5-1809

T Tamura, M Hayakawa and K Hatano Institute for Fermentation, Osaka, 2-17-85 Juso-honmachi Yodogawa-ku, Osaka 532-8686, Japan Four motile spored strains were isolated from soil samples collected in Japan. The cultures formed long, narrow sporangia on short sporangiophores directly on the substrate mycelium. The sporangia develop singly or in clusters above the surface of the substrate. Each sporangium contains a single row of six or more motile spores. Glutamic acid, glucosamine, glycine, alanine and 3-OH-diaminopimelic acid are present in the cell wall; the whole-cell sugars are 3-O-methylmannose, rhamnose, mannose, arabinose, galactose, xylose and glucose; and the predominant menaquinones are 10(H(4)), 10(H(6)) and 10(H(8)). The diagnostic phospholipid is phosphatidylethanolamine. The acyl type of the muramic acid is glycolyl. The G+C content is 71 mol%. Mycolic acids are absent. The chemotaxonomic data indicate that these strains belong to the family Micromonosporaceae. Analysis of 16S rDNA sequences suggested that these organisms fall into a distinct clade within the family Micromonosporaceae for which a new genus, Virgosporangium gen. nov., is proposed containing the species Virgosporangium ochraceum sp. nov. (strains YU655-43(T), YU793-41 and YU794-41) and Virgosporangium aurantiacum sp. nov. (strain YU438-5(T)).