Low-intensity pulsed ultrasound prompts tissue-engineered bone formation after implantation surgery

• Published in: Chinese medical journal Vol. 127; no. 4; pp. 669 - 674
• Main Authors: Wang, Juyong, Wang, Juqiang, Yoshinori, Asou, Paul, Fu, Shen, Huiliang, Chen, Jiani, Sotome, Shinichi, Liu, Zhao, Shinomiya, Kenichi
• Format: Journal Article
• Language: English
• Published: China Department of Orthopaedic Surgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, China%Department of Orthopaedics, First Hospital of China Medical University, Shenyang, Liaoning 110001, China%Department of Orthopaedic Surgery, Graduate School, Tokyo Medical and Dental University, Tokyo 1138519, Japan%Department of Neurological Surgery, Wayne State University School of Medicine, Detroit, MI 48201, USA%Department of Orthopaedic Surgery, Graduate School, Tokyo Medical and Dental University, Tokyo 1138519, Japan 2014; COE Program for Frontier Research on Molecular Destruction and Reconstruction of Tooth and Bone, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 1138519, Japan
• Subjects: Animals; Bone Marrow Cells - physiology; Bone Transplantation; Calcium Phosphates - pharmacology; Male; Osteogenesis - physiology; Rats; Stromal Cells; Tissue Engineering - methods; Transplantation, Isogeneic; Ultrasonics - methods; 低强度; 植入物; 生物反应器; 组织工程化; 脉冲超声波; 骨形成; 骨髓基质干细胞; 骨髓基质细胞; β-tricalcium phosphate; bone formation; low-intensity pulsed ultrasound; marrow stromal cells
• ISSN: 0366-6999; 2542-5641
• DOI: 10.3760/cma.j.issn.0366-6999.20131006

Background A practical problem impeding clinical translation is the limited bone formation seen in artificial bone grafts.Low-pressure/vacuum seeding and dynamic culturing in bioreactors have led to a greater penetration into the scaffolds,enhanced production of bone marrow cells,and improved tissue-engineered bone formation.The goal of this study was to promote more extensive bone formation in the composites of porous ceramics and bone marrow stromal cells （BMSCs）.Methods BMSCs/β-tricalcium phosphate （β-TCP） composites were subcultured for 2 weeks and then subcutaneously implanted into syngeneic rats that were split into a low-intensity pulsed ultrasound （LIPUS） treatment group and a control group.These implants were harvested at 5,10,25,and 50 days after implantation.The samples were then biomechanically tested and analyzed for alkaline phosphate （ALP） activity and osteocalcin （OCN） content and were also observed by light microscopy.Results The levels of ALP activity and OCN content in the composites were significantly higher in the LIPUS group than in the control group.Histomorphometric analysis revealed a greater degree of soft tissue repair,increased blood flow,better angiogenesis,and more extensive bone formation in the LIPUS groups than in the controls.No significant difference in the compressive strength was found between the two groups.Conclusion LIPUS treatment appears to enhance bone formation and angiogenesis in the BMSCs/β3-TCP composites.