Protective properties of ginsenoside Rb3 against UV-B radiation-induced oxidative stress in HaCaT keratinocytes

• Published in: Bioscience, biotechnology, and biochemistry Vol. 80; no. 1; pp. 95 - 103
• Main Authors: Oh, Sun-Joo, Oh, Yuri, Ryu, In Wang, Kim, Kyunghoon, Lim, Chang-Jin
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 2016
• Subjects: Cell Line, Transformed; Enzyme Precursors - antagonists & inhibitors; Enzyme Precursors - genetics; Enzyme Precursors - metabolism; Gene Expression Regulation; ginsenoside Rb3; Ginsenosides - pharmacology; glutathione; Glutathione - agonists; Glutathione - metabolism; Humans; Keratinocytes - drug effects; Keratinocytes - radiation effects; Matrix Metalloproteinase 2 - genetics; Matrix Metalloproteinase 2 - metabolism; Matrix Metalloproteinase 9 - genetics; Matrix Metalloproteinase 9 - metabolism; Oxidation-Reduction; oxidative stress; Oxidative Stress - drug effects; reactive oxygen species; Reactive Oxygen Species - antagonists & inhibitors; Reactive Oxygen Species - metabolism; Signal Transduction; Skin Aging - drug effects; Skin Aging - radiation effects; Sunscreening Agents - pharmacology; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism; Ultraviolet Rays - adverse effects; UV-B radiation; UV-B radiation; glutathione; ginsenoside Rb3; oxidative stress; reactive oxygen species
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1080/09168451.2015.1075862

This work aimed to evaluate the skin anti-photoaging properties of ginsenoside Rb3 (Rb3), one of the main protopanaxdiol-type ginsenosides from ginseng, in HaCaT keratinocytes. The skin anti-photoaging activity was assessed by analyzing the levels of reactive oxygen species (ROS), pro-matrix metalloproteinase-2 (proMMP-2), pro-matrix metalloproteinase-9 (proMMP-9), total glutathione (GSH), and superoxide dismutase (SOD) activity as well as cell viability in HaCaT keratinocytes under UV-B irradiation. When HaCaT keratinocytes were exposed to Rb3 prior to UV-B irradiation, Rb3 exhibited suppressive activities on UV-B-induced ROS, proMMP-2, and proMMP-9 enhancements. On the contrary, Rb3 displayed enhancing activities on UV-B-reduced total GSH and SOD activity levels. Rb3 could not interfere with cell viabilities in UV-B-irradiated HaCaT keratinocytes. Rb3 plays a protective role against UV-B-induced oxidative stress in human HaCaT keratinocytes, proposing its potential skin anti-photoaging properties. Effects of Rb3 on the UV-B-induced proMMP-2 (A) and proMMP-9 (B) activities in conditioned media of HaCaT keratinocytes. In (C), the equal loading was shown.