iPSC lines that do not silence the expression of the ectopic reprogramming factors may display enhanced propensity to genomic instability

• Published in: Cell research Vol. 20; no. 10; pp. 1092 - 1095
• Main Authors: Ramos-Mejia, Veronica, Muñoz-Lopez, Martin, Garcia-Perez, Jose L, Menendez, Pablo
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.10.2010; Nature Publishing Group
• Subjects: 631/208/176/2016; 631/208/211; 631/532/2064/2158; 631/532/2435; Biomedical and Life Sciences; Cell Biology; Cellular Reprogramming; commentary; Data processing; Gene Silencing; Genomic Instability; Genomics; Induced Pluripotent Stem Cells - metabolism; Inhibitory postsynaptic potentials; Karyotyping; Lentivirus - genetics; Life Sciences; Scientists; Somatic cells; Standardization; Stem cells; 体细胞; 基因组不稳定性; 干细胞因子; 标准化; 重新编程; 重编程; gene silencing; iPSCs; reprogramming factors; genomic stability
• ISSN: 1001-0602; 1748-7838
• DOI: 10.1038/cr.2010.125

Here, we provide data suggesting that the absence of silencing of the ectopic reprogramming factors used to reprogram somatic cells to induced pluripotent stem cells （iPSCs） may predispose iPSCs to genomic instability. We encourage stem cell scientists to undertake an extensive characterization and standardization of much larger cohorts of iPSC lines in order to set up rigorous criteria to define safe and stable bonafide iPSCs.