Characterisation of a starch-hydrolysing enzyme of Aspergillus niger

• Published in: Applied microbiology and biotechnology Vol. 51; no. 5; pp. 673 - 675
• Main Authors: Suresh, C, Dubey, A.K, Srikanta, S, Umesh Kumar, S, Karanth, N.G
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.05.1999; Springer Nature B.V
• Subjects: amylolytic enzymes; Aspergillus niger; Aspergillus niger - enzymology; Bacteria; Bacteriology; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; Enzymes; Fundamental and applied biological sciences. Psychology; Hydrogen-Ion Concentration; Hydrolases - isolation & purification; Hydrolases - metabolism; hydrolysis; Miscellaneous; Mission oriented research; starch; Starch - metabolism; Temperature; Brewery; Enzyme; Starch; Industrial application; Thermal stability; Fungi; Hydrolysis; Aspergillus niger; Substrate specificity; Bakery industry; Fungi Imperfecti; Amylolytic enzyme; Physicochemical properties; Thallophyta
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s002530051450

A UV-induced mutant strain of Aspergillus niger (CFTRI-1105-U9) overproduced a starch-hydrolysing enzyme with properties characteristically different from the known amylases of the fungus. The purified enzyme of 4.0 pI had an apparent molecular mass of 125 kDa and it dextrinised starch and then saccharified the dextrins. Patterns of the enzyme activity on starch, resulting in glucose at 60 degrees C and glucose, maltose and maltodextrins at 70 degrees C as primary products, suggested significant applications for the enzyme in starch-processing industries.