Enhanced quality of capacitated spermatozoa from oligoasthenozoospermic men after incubation in test yolk medium

To improve artificially the quality of poor sperm samples to be used in assisted procreation procedures, Test Yolk Buffer (TYB) incubation effect was investigated. After adding the TYB procedure to the standard swim-up technique, a significant (P < 0.001) increase in spermatozoa velocity was obse...

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Bibliographic Details
Published inAndrologia Vol. 25; no. 6; p. 337
Main Authors Ragni, G, Lombroso Finzi, G C, Caccamo, A, Dalla Serra, A, Crosignani, P G
Format Journal Article
LanguageEnglish
Published Germany 01.11.1993
Subjects
Buffers
Female
Glucose
Humans
Insemination, Artificial, Homologous
Male
Oligospermia - physiopathology
Pregnancy
Sperm Capacitation - physiology
Sperm Motility
Tromethamine
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Summary:To improve artificially the quality of poor sperm samples to be used in assisted procreation procedures, Test Yolk Buffer (TYB) incubation effect was investigated. After adding the TYB procedure to the standard swim-up technique, a significant (P < 0.001) increase in spermatozoa velocity was observed, while the other seminal parameters were not affected. Three of 11 couples who had not had success after three intraperitoneal inseminations (IPI) with sperm prepared by the usual swim-up technique obtained pregnancies after IPI with TYB-incubated spermatozoa. These preliminary results suggest that TYB incubation for capacitated spermatozoa be employed in the assisted procreation strategies applied for male infertility.
ISSN:0303-4569
DOI:10.1111/j.1439-0272.1993.tb02738.x