Yoghurt impacts on the excretion of phenolic acids derived from colonic breakdown of orange juice flavanones in humans

• Published in: Molecular nutrition & food research Vol. 53; no. S1; pp. S68 - S75
• Main Authors: Roowi, Suri, Mullen, William, Edwards, Christine A, Crozier, Alan
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley-VCH Verlag 01.05.2009; WILEY-VCH Verlag; WILEY‐VCH Verlag
• Subjects: Adult; Beverages - analysis; Carboxylic Acids - urine; Citrus; Citrus sinensis - chemistry; Colon - microbiology; Colonic catabolism; Flavanones; Flavanones - administration & dosage; Flavanones - analysis; Flavanones - metabolism; Fruit - chemistry; Gas Chromatography-Mass Spectrometry; Hesperidin - analysis; Hesperidin - metabolism; Humans; Middle Aged; Orange juice; Urinary phenolic acids; Yoghurt; Yogurt
• ISSN: 1613-4125; 1613-4133
• DOI: 10.1002/mnfr.200800287

Human urine was collected over a 24 h period after the consumption of 250 mL of (i) water, (ii) orange juice, and (iii) orange juice plus 150 mL of full fat natural yoghurt. The orange juice contained 168 μmol of hesperetin-7-O-rutinoside and 18 μmol of naringenin-7-O-rutinoside. GC-MS analysis of the urine identified nine phenolic acids, five of which, 3-hydroxyphenylacetic acid, 3-hydroxyphenylhydracrylic acid, dihydroferulic acid, 3-methoxy-4-hydroxyphenylhydracrylic acid and 3-hydroxyhippuric acid, were associated with orange juice consumption indicating that they were derived from colonic catabolism of hesperetin-7-O-rutinoside. The overall 0-24 h excretion of the five phenolic acids was 6.7 ± 1.8 μmol after drinking water and this increased significantly (p < 0.05) to 62 ± 18 μmol, equivalent to 37% of the ingested flavanones, following orange juice consumption. When the orange juice was ingested with yoghurt excretion fell back markedly to 9.3 ± 4.4 μmol. This was not due to a difference in mouth to caecum transit time, as measured with breath hydrogen production, though possibly there may have been a slowing of the bulk of the meal reaching the large intestine which may then have altered the catabolism of the flavanones to phenolic acids by the colonic microbiota.