SABER: A computational method for identifying active sites for new reactions
A software suite, SABER (Selection of Active/Binding sites for Enzyme Redesign), has been developed for the analysis of atomic geometries in protein structures, using a geometric hashing algorithm (Barker and Thornton, Bioinformatics 2003;19:1644–1649). SABER is used to explore the Protein Data Bank...
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Published in | Protein science Vol. 21; no. 5; pp. 697 - 706 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Hoboken
Wiley Subscription Services, Inc., A Wiley Company
01.05.2012
Wiley Subscription Services, Inc |
Subjects | |
Online Access | Get full text |
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Summary: | A software suite, SABER (Selection of Active/Binding sites for Enzyme Redesign), has been developed for the analysis of atomic geometries in protein structures, using a geometric hashing algorithm (Barker and Thornton, Bioinformatics 2003;19:1644–1649). SABER is used to explore the Protein Data Bank (PDB) to locate proteins with a specific 3D arrangement of catalytic groups to identify active sites that might be redesigned to catalyze new reactions. As a proof‐of‐principle test, SABER was used to identify enzymes that have the same catalytic group arrangement present in o‐succinyl benzoate synthase (OSBS). Among the highest‐scoring scaffolds identified by the SABER search for enzymes with the same catalytic group arrangement as OSBS were L‐Ala D/L‐Glu epimerase (AEE) and muconate lactonizing enzyme II (MLE), both of which have been redesigned to become effective OSBS catalysts, demonstrated by experiments. Next, we used SABER to search for naturally existing active sites in the PDB with catalytic groups similar to those present in the designed Kemp elimination enzyme KE07. From over 2000 geometric matches to the KE07 active site, SABER identified 23 matches that corresponded to residues from known active sites. The best of these matches, with a 0.28 Å catalytic atom RMSD to KE07, was then redesigned to be compatible with the Kemp elimination using RosettaDesign. We also used SABER to search for potential Kemp eliminases using a theozyme predicted to provide a greater rate acceleration than the active site of KE07, and used Rosetta to create a design based on the proteins identified. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Grant sponsors: National Institute of General Medical Science; Grant number: GM 75962; Grant sponsor: National Institutes of Health; Grant numbers: T32 GM008496-16; Grant sponsors: Department of Defense, Defense Advanced Research Projects Agency. |
ISSN: | 0961-8368 1469-896X |
DOI: | 10.1002/pro.2055 |