Use of reconstructed small intestine submucosa for urinary tract replacement

We used reconstructed SIS (ReSIS), a photocrosslinked biomaterial, to create grafts in various shapes and sizes. Sheets of ReSIS were placed in 14 swine to repair bladder defects, and ReSIS tubes were placed in six swine to replace a segment of excised ureter. Histologic analysis of the bladder repa...

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Bibliographic Details
Published inASAIO journal (1992) Vol. 46; no. 3; p. 268
Main Authors Xie, H, Shaffer, B S, Wadia, Y, Gregory, K W
Format Journal Article
LanguageEnglish
Published United States 01.05.2000
Subjects
Animals
Artificial Organs
Female
Intestinal Mucosa - cytology
Intestinal Mucosa - transplantation
Intestine, Small - cytology
Intestine, Small - transplantation
Kidney Function Tests
Reconstructive Surgical Procedures - methods
Swine
Ureter - cytology
Ureter - surgery
Urinary Bladder - cytology
Urinary Bladder - surgery
Urothelium - cytology
Urothelium - surgery
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Summary:We used reconstructed SIS (ReSIS), a photocrosslinked biomaterial, to create grafts in various shapes and sizes. Sheets of ReSIS were placed in 14 swine to repair bladder defects, and ReSIS tubes were placed in six swine to replace a segment of excised ureter. Histologic analysis of the bladder repair revealed transitional urothelial cells lining the ReSIS by 1 week. After 2 weeks, fibroblasts and mononuclear cells had infiltrated the ReSIS, neovascularization had occurred, and the urothelial lining was more complex, containing multiple cell layers. After 4 weeks, a definite submucosa was present and the ReSIS was starting to degrade. An initial muscular regeneration was demonstrated at 12 weeks. No foreign body reaction, calcification, or sedimentation was noted in any animal. The ureteral implants showed identical histologic changes, without obstruction or leakage of the replaced segment. The ReSIS allowed rapid urothelial regeneration, ingrowth of new blood vessels, and the orderly deposition and organization of new collagen. Our study demonstrates that the photocrosslinking technique used to create larger sheets and tubes of this biomaterial (ReSIS) does not detract from the positive attributes of the SIS and should improve its usefulness in accomplishing larger bladder augmentations and ureter replacements.
ISSN:1058-2916
DOI:10.1097/00002480-200005000-00005