Sequential expression of CD34 and CD33 antigens on myeloid colony-forming cells

• Published in: European journal of haematology Vol. 42; no. 2; p. 143
• Main Authors: Bühring, H J, Asenbauer, B, Katrilaka, K, Hummel, G, Busch, F W
• Format: Journal Article
• Language: English
• Published: England 01.02.1989
• Subjects: Antigens, CD; Antigens, CD34; Antigens, Differentiation - analysis; Antigens, Differentiation, Myelomonocytic - analysis; Bone Marrow - immunology; Colony-Forming Units Assay; Hematopoietic Stem Cells - immunology; Humans; Sialic Acid Binding Ig-like Lectin 3
• ISSN: 0902-4441
• DOI: 10.1111/j.1600-0609.1989.tb01203.x

The expression of CD33 and CD34 antigens on human bone marrow cells was examined by fluorescence-activated cell sorting and colony assays. A marked difference of antigen expression was observed on sorted progenitors of the granulocyte/macrophage lineage (CFU-GM) with respect to enrichment and maturation status. Single-color cell sorting revealed no difference of enrichment by anti-CD33 antibody between day-7 and d-14 progenitors, while anti-CD34 antibody preferentially enriched d-14 colony-forming units (CFU). By two-color cell sorting it could be shown that enrichment of d-14 CFU-GM occurred mostly in the CD34+CD33- and to a lesser extent in the double-positive fraction. In contrast, there was no difference in degree of enrichment between d-7 and d-14 CD34-CD33+ CFU-GM. From these data we conclude that, during early myelopoiesis, CD34 antigens are gradually lost while CD33 antigens are acquired.