Distribution of vacA genotypes in Helicobacter pylori strains isolated from Brazilian adult patients with gastritis, duodenal ulcer or gastric carcinoma

• Published in: FEMS immunology and medical microbiology Vol. 33; no. 3; pp. 173 - 178
• Main Authors: Ashour, Abdussalam A.R., Magalhães, Paula P., Mendes, Edilberto N., Collares, Guilherme B., de Gusmão, Valquíria R., Queiroz, Dulciene M.M., Nogueira, Ana Margarida M.F., Rocha, Gifone A., de Oliveira, Celso A.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 12.07.2002; Blackwell; Oxford University Press
• Subjects: Adolescent; Adult; Aged; Antigens, Bacterial; Bacterial diseases; Bacterial diseases of the digestive system and abdomen; Bacterial genetic diversity; Bacterial Proteins - genetics; Bacterial Typing Techniques; Bacteriology; Biological and medical sciences; Brazil - epidemiology; cagA; Duodenal Ulcer - epidemiology; Duodenal Ulcer - microbiology; Epidemiology; Female; Fundamental and applied biological sciences. Psychology; Gastric cancer; Gastritis; Gastritis - epidemiology; Gastritis - microbiology; Genotype; Genotypes; Genotyping; Helicobacter Infections - epidemiology; Helicobacter Infections - microbiology; Helicobacter pylori; Helicobacter pylori - classification; Helicobacter pylori - genetics; Helicobacter pylori - isolation & purification; Human bacterial diseases; Humans; Infectious diseases; Male; Medical sciences; Microbiology; Middle Aged; Mixed infection; Polymerase Chain Reaction; Stomach Neoplasms - epidemiology; Stomach Neoplasms - microbiology; Strains (organisms); Ulcers; vacA; Brazil; Bacterial genetic diversity; Human; Stomach; Carcinoma; Mixed infection; Spirillales; Duodenum; Spirillaceae; Genotype; Malignant tumor; Gastritis; Infection; Pathogenicity; Helicobacter pylori; Bacteriosis; Molecular epidemiology; Digestive diseases; Bacteria; Intestinal disease; Ulcer; Clinical isolate
• ISSN: 0928-8244; 1574-695X; 2049-632X
• DOI: 10.1111/j.1574-695X.2002.tb00588.x

Abstract This PCR-based analysis is the first molecular epidemiological study in Brazil testing Helicobacter pylori cagA and vacA distribution in adults with gastric complaints, that includes a large number of carcinoma patients. Multiple-strain infection was identified in 11/13.4% patients. vacA s1-m1 and cagA+ genotypes were the most common in patients with a non-mixed infection. All vacA s1 strains were s1b, so subtyping s1 strains was not useful. vacA s1b-m1 and cagA+ strains were associated with higher prevalence of peptic ulcer and gastric carcinoma than vacA s2-m2 and cagA− ones. In conclusion, cagA and vacA genotyping may have clinical relevance in Brazil.