The partially degraded lipopolysaccharide of Burkholderia cepacia and ornithine-containing lipids derived from some Gram-negative bacteria are useful complex lipid adjuvants

• Published in: FEMS immunology and medical microbiology Vol. 34; no. 3; pp. 173 - 179
• Main Authors: Kawai, Yohko, Watanabe, Mineo, Matsuura, Motohiro, Nishijima, Masahiro, Kawahara, Kazuyoshi
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 15.11.2002; Blackwell; Oxford University Press
• Subjects: Adjuvant; Adjuvants; Adjuvants, Immunologic - pharmacology; Adjuvants, Immunologic - therapeutic use; Alum Compounds - chemistry; Alum Compounds - metabolism; Aluminum; Aluminum hydroxide; Animals; Antigens; Bacteria; Bacteriology; Biological and medical sciences; Burkholderia cepacia; Burkholderia cepacia - chemistry; Burkholderia cepacia - immunology; Burkholderia cepacia - isolation & purification; Diphtheria; Diphtheria Toxoid - immunology; Fundamental and applied biological sciences. Psychology; Gram-negative bacteria; Gram-Negative Bacteria - chemistry; Gram-Negative Bacteria - classification; Gram-Negative Bacteria - metabolism; IgG antibody; Immune system; Immunoglobulin G; Interleukin-6 - biosynthesis; Lipid Metabolism; Lipids; Lipids - chemistry; Lipids - pharmacology; Lipopolysaccharides; Lipopolysaccharides - isolation & purification; Lipopolysaccharides - metabolism; Lipopolysaccharides - pharmacology; Mice; Mice, Inbred BALB C; Mice, Inbred C3H; Microbiology; Ornithine; Ornithine - analysis; Ornithine‐containing lipid; Partially degraded lipopolysaccharide; Pertussis; Staphylococcal Toxoid - immunology; Tetanus; Tetanus Toxoid - immunology; Toxicity; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Partially degraded lipopolysaccharide; Ornithine-containing lipid; Adjuvant; Toxin; Immunization; Immunological adjuvant; Burkholderia cepacia; Lipopolysaccharide; Bacteria; Lipids; Subcutaneous administration; Ornithine; Humoral immunity; Gram negative bacteria
• ISSN: 0928-8244; 1574-695X; 2049-632X
• DOI: 10.1111/j.1574-695X.2002.tb00621.x

Abstract The partially degraded lipopolysaccharide of Burkholderia cepacia (LPSdegr) and the ornithine-containing lipids were purified from some bacteria. The substances were developed as complex lipid adjuvants, because they have weak toxicity and are able to activate the immune systems of the living body. After various toxoid antigens such as pertussis toxoid, diphtheria toxoid and tetanus toxoid were mixed with the complex lipid adjuvants, the mixtures were administered to mice subcutaneously. Antitoxoid IgG antibody titers in the serum were measured several times over 3 months. The efficacy of the LPSdegr as adjuvant was almost as high as that of the ornithine-containing lipids, and it was almost equal to that of the aluminum hydroxide adjuvant (Alum), which is generally used as a vaccine adjuvant.