The quantification of fetomaternal hemorrhage by an enzyme-linked antibody test with glutaraldehyde fixation

A modified enzyme-linked antiglobulin test (ELAT) for quantifying D-positive red blood cells in the circulation of D-negative pregnant women is described. The antibody-antigen bond was stabilized and the problem of hemolysis eliminated by the use of 0.05% glutaraldehyde. The r value for the standard...

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Bibliographic Details
Published inVox sanguinis Vol. 63; no. 4; p. 268
Main Authors Greenwalt, T J, Dumaswala, U J, Domino, M M
Format Journal Article
LanguageEnglish
Published England 01.01.1992
Subjects
Enzyme-Linked Immunosorbent Assay - methods
Female
Fetomaternal Transfusion - diagnosis
Glutaral
Humans
Immunoglobulin G - analysis
Pregnancy
Rh-Hr Blood-Group System
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Summary:A modified enzyme-linked antiglobulin test (ELAT) for quantifying D-positive red blood cells in the circulation of D-negative pregnant women is described. The antibody-antigen bond was stabilized and the problem of hemolysis eliminated by the use of 0.05% glutaraldehyde. The r value for the standardization curves for measuring cord RBC in mixtures was 0.98; coefficient of variation 5.8%. Reproducible quantitation of 0.125% D-positive cord RBC mixed with D-negative RBC was demonstrated. In comparative studies of artificial mixtures, Kleihauer-Betke results for more than 1% cord RBC were 50-100% greater than the true values. In several examples of large fetomaternal hemorrhages, the ELAT results matched the clinical data more closely than the Kleihauer-Betke based estimates. 2 ml of fetal RBC in a 1,600-ml red cell mass can be quantified using the modified ELAT.
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1992.tb01233.x