High MHC class I expression correlates with slow growth in UV-induced skin carcinomas in hairless mice
An experiment was set up to investigate the relationship, if any, between cell surface MHC class I expression and the growth rate for skin tumors induced by two different UV radiation regimens in hairless mice. Two groups of 20 hairless mice were each irradiated with either a UVA radiation source (2...
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Published in | APMIS : acta pathologica, microbiologica et immunologica Scandinavica Vol. 106; no. 7-12; pp. 1101 - 1107 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Publishing Ltd
01.11.1998
Blackwell |
Subjects | |
Online Access | Get full text |
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Summary: | An experiment was set up to investigate the relationship, if any, between cell surface MHC class I expression and the growth rate for skin tumors induced by two different UV radiation regimens in hairless mice. Two groups of 20 hairless mice were each irradiated with either a UVA radiation source (2 SED per session) or broad‐spectrum UV radiation (UVB) (8.1 SED per session) 5 days a week during the entire experiment. In the UVA group, 17 out of 20 animals developed tumors, and 10 of these grew to a diameter of ≥5 mm. In the UVB group, 19 out of 20 animals developed tumors, and 15 of these grew to a diameter of ≥5 mm. The tumor induction time, i.e. the time from the start of UV treatment to tumor appearance, was found to be significantly longer (p<0.01) in the UVA than in the UVB group. This is in accordance with previous findings. Of the 25 tumors growing to a diameter of ≥5 mm, 11 were established as cultured cell lines (4 UVA and 7 UVB tumors). These uncloned cell lines were analyzed for surface expression of major histocompatibility complex class I by FACS analysis. There was a clear correlation between high MHC class I expression and slow growth of the individual tumors (p<0.05). This suggests a role for the MHC class I governed, i.e. cytotoxic T‐cell‐mediated, reactions in deciding the fate of UV‐induced skin cancers. No correlation was found between MHC class I expression and tumor induction time. |
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Bibliography: | ark:/67375/WNG-B7SCP98S-5 ArticleID:APM1101 istex:F8314235115CE184BE5A8F28D5831E68F09781C9 Anne‐Marie Engel and Inge Marie Svane have contributed equally to this work. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0903-4641 1600-0463 |
DOI: | 10.1111/j.1699-0463.1998.tb00265.x |