On the Hormonal Nature of the Stimulatory Effect of High Incubation Temperatures on Germination of Dormant Sorghum (S. bicolor) Caryopses

• Published in: The New phytologist Vol. 160; no. 2; pp. 371 - 377
• Main Authors: Benech-Arnold, Roberto L., Enciso, Silvina, Sánchez, Rodolfo A., Rodríguez, M. Verónica
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science 01.11.2003; Blackwell Science Ltd
• Subjects: abscisic acid; Dormancy; Embryos; Gas temperature; Germination; Gibberellins; Grains; High temperature; hormonal regulation; Low temperature; Panicles; pre‐harvest sprouting; seed dormancy; Sorghum; temperature; germination; pre-harvest sprouting; seed dormancy; sorghum; temperature; abscisic acid; gibberellins; hormonal regulation
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1046/j.1469-8137.2003.00900.x

• High incubation temperatures (i.e. 30°C) stimulate the germination of dormant sorghum grains. To test the hormonal nature of this response, experiments were carried out with two varieties with contrasting dormancy at harvest: Redland B2 (low dormancy, high germination percentages attained under a wide incubation thermal range) and IS 9530 (high dormancy, high germination percentages attained only at 30°C). • Redland B2 grains with reduced GA content (paclobutrazol-treated) reached high germination temperatures (c. 100%) only when incubated at 30°C. By contrast, IS 9530 grains with reduced ABA content (fluridone-treated) reached 100% germination at 30, 25, 20 and 15°C. • Incubation temperatures did not alter embryo responsiveness to ABA, nor did it modify the pattern of changes in embryo ABA content throughout incubation. Low GA3 concentrations (0.1 μM) were required to totally overcome the inhibition imposed by ABA in embryos incubated at 30°C; by contrast, even the highest GA3 concentrations used (1000 μM) were not able to revert ABA inhibition in embryos incubated at 15°C. • These results show the hormonal nature of the stimulatory effect of high incubation temperatures, and suggest that this effect is mediated by an increase in tissue responsiveness to GAs.