Modulation of actin isoform expression before the transition from experimental compensated pressure-overload cardiac hypertrophy to decompensation
1 Department of Evolutionary and Functional Biology, Physiology Section, University of Parma, Italy; and 2 Departments of Pathology and Immunology and 3 Clinical Pathology, University of Geneva, Switzerland Submitted 2 October 2008 ; accepted in final form 21 February 2009 In a rat model of long-las...
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Published in | American journal of physiology. Heart and circulatory physiology Vol. 296; no. 5; pp. H1625 - H1632 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
American Physiological Society
01.05.2009
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Subjects | |
Online Access | Get full text |
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Summary: | 1 Department of Evolutionary and Functional Biology, Physiology Section, University of Parma, Italy; and 2 Departments of Pathology and Immunology and 3 Clinical Pathology, University of Geneva, Switzerland
Submitted 2 October 2008
; accepted in final form 21 February 2009
In a rat model of long-lasting pressure-overload hypertrophy, we investigated whether changes in the relative expression of myocardial actin isoforms are among the early signs of ventricular mechanical dysfunction before the transition toward decompensation. Forty-four rats with infrarenal aortic banding (AC rats) were studied. Hemodynamic parameters were measured 1 mo (AC 1 group; n = 20) or 2 mo (AC 2 ; n = 24) after aortic ligature. Then subgroups of AC 1 and AC 2 left ventricles (LV) were used to evaluate 1 ) LV anatomy and fibrosis (morphometry), 2 ) expression levels (immunoblotting) and spatial distribution (immunohistochemistry) of alpha-skeletal actin ( -SKA), alpha-cardiac actin ( -CA), and alpha-smooth muscle actin ( -SMA), and 3 ) cell mechanics and calcium transients in enzimatically isolated myocytes. Although the two AC groups exhibited a comparable degree of hypertrophy (+30% in LV mass; +20% in myocyte surface) and a similar increase in the amount of fibrosis compared with control animals (C group; n = 22), a worsening of LV mechanical performance was observed only in AC 2 rats at both organ and cellular levels. Conversely, AC 1 rats exhibited enhanced LV contractility and preserved cellular contractile behavior associated with increased calcium transients. Alpha-SKA expression was upregulated (+60%) in AC 1 . In AC 2 ventricles, prolonged hypertension also induced a significant increase in -SMA expression, mainly at the level of arterial vessels. No significant differences among groups were observed in -CA expression. Our findings suggest that -SKA expression regulation and wall remodeling of coronary arterioles participate in the development of impaired kinetics of contraction and relaxation in prolonged hypertension before the occurrence of marked histopathologic changes.
aortic ligature; hypertension
Address for reprint requests and other correspondence: D. Stilli, Dept. of Evolutionary and Functional Biology, Physiology Section, Univ. of Parma, V. le G. P. Usberti 11/A, I-43100, Italy (e-mail: donatella.stilli{at}unipr.it ) |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0363-6135 1522-1539 |
DOI: | 10.1152/ajpheart.01057.2008 |