Dietary arginine supplementation enhances immune responses to inactivated Pasteurella multocida vaccination in mice

• Published in: British journal of nutrition Vol. 109; no. 5; pp. 867 - 872
• Main Authors: Ren, Wenkai, Zou, Lingxiu, Li, Nengzhang, Wang, Yan, Liu, Gang, Peng, Yuanyi, Ding, Jiannan, Cai, Lichuang, Yin, Yulong, Wu, Guoyao
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 14.03.2013
• Subjects: Adjuvants, Immunologic - administration & dosage; Amino acids; Animals; Antibodies, Bacterial - blood; Arginine - administration & dosage; Bacteria; Bacterial Vaccines - immunology; Biological and medical sciences; Cytokines - blood; Dietary Supplements; Feeding. Feeding behavior; Female; Fundamental and applied biological sciences. Psychology; Glutathione Peroxidase - blood; Immune system; Immunity, Active - immunology; Immunization; Mice; Nutritional Immunology; Pasteurella Infections - prevention & control; Pasteurella multocida; Pasteurella multocida - immunology; Rodents; Vaccines, Inactivated - immunology; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Adjuvants; Arginine; Nutrition; Pasteurella multocida; Immune response; Vaccination; Rodentia; Feeding; Arginine: Pasteurella multocida; Vertebrata; Mammalia; Mouse; Animal; Supplementation
• ISSN: 0007-1145; 1475-2662
• DOI: 10.1017/S0007114512002681

The present study was conducted to determine the adjuvant effect of arginine in mice immunised with inactivated vaccine. Mice immunised with an inactivated Pasteurella multocida vaccine and fed diets supplemented with 0·2 % (vaccine-0·2 %) or 0·5 % (vaccine-0·5 %) arginine exhibited 100 % protection from a challenge with P. multocida serotype A (CQ2) at a dose of 4·4 × 105 colony-forming units (2LD50; median lethal dose), when compared with mice receiving no arginine supplementation. Meanwhile, antibody titres in the vaccine-0·2 % arginine group were much higher than those in the vaccine-oil adjuvant group before challenge and at 36 h post-infection. Furthermore, immunisation with the inactivated vaccine and dietary supplementation with 0·2 % arginine increased serum levels of glutathione peroxidase, in comparison with immunisation with the inactivated vaccine and an oil adjuvant. Collectively, dietary arginine supplementation confers an immunostimulatory effect in mice immunised with the inactivated P. multocida vaccine. The present results also indicate that optimal supplemental doses of arginine are 0·2–0·5 % in the mouse model.