Synergistic interaction between Beauveria bassiana- and Bacillus thuringiensis tenebrionis-based biopesticides applied against field populations of Colorado potato beetle larvae

• Published in: Journal of invertebrate pathology Vol. 90; no. 3; pp. 139 - 150
• Main Authors: Wraight, S.P., Ramos, M.E.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.11.2005; Elsevier
• Subjects: Animals; application rate; Ascomycota; Bacillus thuringiensis; Bacillus thuringiensis subsp. tenebrionis; Bacillus thuringiensis tenebrionis; bacterial insecticides; Beauveria; Beauveria bassiana; Biological and medical sciences; Biological control; Biopesticides; Coleoptera - microbiology; Colorado potato beetle; Control; Curculionidae; Fundamental and applied biological sciences. Psychology; insect control; Interaction; Larva - microbiology; larvae; Leptinotarsa decemlineata; mycoinsecticides; Pest Control, Biological - methods; pesticide mixtures; Phytopathology. Animal pests. Plant and forest protection; population density; potatoes; Protozoa. Invertebrates; Solanum tuberosum; Synergism; New York; Beauveria bassiana; Bacillus thuringiensis tenebrionis; Leptinotarsa decemlineata; Colorado potato beetle; Interaction; Biopesticides; Synergism; Chrysomelidae; Coleoptera; Insecta; Biological control; Bacillus thuringiensis; Bacillaceae; Fungi; Pest; Microbiological control; Bacillales; Bacteria; Fungi Imperfecti; Biopesticide; Thallophyta; Parasite; Mycoparasite; Larva; Arthropoda; Invertebrata; Microbial insecticide
• ISSN: 0022-2011; 1096-0805
• DOI: 10.1016/j.jip.2005.09.005

Commercial biopesticides based on the fungal pathogen Beauveria bassiana strain GHA and the bacterial pathogen Bacillus thuringiensis tenebrionis were applied alone and in combination (tank mixed) against larval populations of the Colorado potato beetle, Leptinotarsa decemlineata, in small plots of potatoes over three field seasons. Interactions between the two products were evaluated in terms of pest-control efficacy. B. bassiana (formulated as Mycotrol) was applied at low and medium label rates of 1.25 and 2.5 × 10 13 conidia/ha, and B. thuringienis (formulated as Novodor) was applied at low and high label rates of 40.3 and 120.8 × 10 6 Leptinotarsa units/ha. Two weekly applications of the bacterial pesticide alone provided 50–85% control of beetle larvae within 14 days after the initial application, while applications of the mycopesticide alone produced no greater than 25% control. Maximum control, in nearly all tests, was produced by the combination of the two products. The combined treatments produced a statistically significant 6–35% greater reduction in larval populations than would have been predicted had the two biopesticides acted independently. This low-level synergistic interaction was observed during all field seasons and resulted from combinations at all rates, including, in one of two tests, the low rates of each product. These results indicate that B. thuringiensis and B. bassiana have strong potential for integrated biologically based management of Colorado potato beetle.