Neuraminidase Inhibitors from the Fermentation Broth of Phellinus linteus

• Published in: Mycobiology Vol. 42; no. 2; pp. 189 - 192
• Main Authors: Hwang, Byung Soon, Lee, Myeong-Seok, Lee, Seung Woong, Lee, In-Kyoung, Seo, Geon-Sik, Choi, Hwa Jung, Yun, Bong-Sik
• Format: Journal Article
• Language: English
• Published: Korea (South) The Korean Society of Mycology 01.06.2014; 한국균학회
• Subjects: Research Note; 생물학; Phellinus linteus; Anti-influenza agent; 4-(3,4-Dihydroxyphenyl)-3-buten-2-one; Neuraminidase inhibitor; Inotilone
• ISSN: 1229-8093; 2092-9323
• DOI: 10.5941/MYCO.2014.42.2.189

During a search for neuraminidase inhibitors derived from medicinal fungi, we found that the fermentation broth of Phellinus linteus exhibited potent neuraminidase inhibitory activity. Through bioassay-guided fractionation, two active compounds were purified from the ethyl acetate-soluble portion of the fermentation broth of P. linteus. These structures were identified as inotilone (1) and 4-(3,4-dihydroxyphenyl)-3-buten-2-one (2) by spectroscopic methods. Compounds 1 and 2 inhibited H1N1 neuraminidase activity with IC50 values of 29.1 and 125.6 µM, respectively, in a dose-dependent manner. They also exhibited an antiviral effect in a viral cytopathic effect reduction assay using MDCK cells. These results suggest that compounds 1 and 2 from the culture broth of P. linteus would be good candidates for the prevention and therapeutic strategies towards viral infections.