Broccoli sprouts juice prevents lens protein aggregation in streptozotocin-induced diabetic rat

• Published in: International journal of ophthalmology Vol. 12; no. 9; pp. 1380 - 1385
• Main Author: Puspitasari, Ayu
• Format: Journal Article
• Language: English
• Published: International Journal of Ophthalmology Press 18.09.2019; Press of International Journal of Ophthalmology (IJO PRESS)
• Subjects: Basic Research; broccoli sprouts; diabetic cataract; lens αb-crystallin; oxidative stress; rat
• ISSN: 2222-3959; 2227-4898
• DOI: 10.18240/ijo.2019.09.02

AIMTo investigate the effect of broccoli sprouts juice in preventing lens protein aggregation in diabetic rat model. METHODSTotally 25 male Wistar rats were divided into negative control group, diabetic group without juice treatment as positive control and diabetic group given broccoli sprouts juice 0.25, 0.5 and 1 g/d for 28d. Diabetic rat model was obtained by administering a single dose streptozotocin 65 mg/kg intraperitoneal. At the end of the study, all rats were examined for fasting blood sugar level (FBS), visual cataract score using slit lamp biomicroscope and lens αB-crystallin expression using Western blot method. Statistical analysis was performed using one way ANOVA with post hoc test, correlation test, and simple linear regression. RESULTSPositive control group had the highest cataract score and lens aggregated αB-crystallin expression. Broccoli sprout juice dose of 1 g/d group had the mildest cataract score, as well as the expression of lens aggregated αB-crystallin compared to treatments groups 1 and 2, opposite to lens native αB-crystallin expression. The broccoli sprout juice groups gave a significant decrease in cataract score, and also in lens aggregated αB-crystallin expression in diabetic rat models (P<0.05). CONCLUSIONBroccoli sprout juice has a significant effect in preventing lens protein aggregation in diabetic rat model. The higher dose gives better visual cataract scores, lower lens aggregated αB-crystallin expression and higher lens native αB-crystallin expression.