Influence of dietary cholesterol on polyunsaturated fatty acid composition, fluidity and membrane-bound enzymes in liver microsomes of rats fed olive and fish oil

• Published in: Biochimie Vol. 74; no. 6; pp. 551 - 556
• Main Authors: Muriana, F.J.G., Ruiz-Gutierrez, V., Vazquez, C.M.
• Format: Journal Article
• Language: English
• Published: Paris Elsevier Masson SAS 01.06.1992; Elsevier
• Subjects: ACEITE DE OLIVA; ACEITES DE PESCADO; ACIDE GRAS INSATURE; ACIDOS GRASOS INSATURADOS; acyl-CoA; Administration, Oral; ALIMENTOS; ANIMAL DE LABORATOIRE; ANIMALES DE LABORATORIO; Animals; biochemical composition; Biological and medical sciences; Biological membranes; CHEMICAL COMPOSITION; CHOLESTEROL; cholesterol acyltransferase; Cholesterol, Dietary - pharmacology; COLESTEROL; COMPOSICION QUIMICA; composition; COMPOSITION CHIMIQUE; desaturase; diets; fatty acid composition; fatty acids; Fatty Acids, Unsaturated - chemistry; Fatty Acids, Unsaturated - metabolism; FISH OILS; Fish Oils - administration & dosage; FOIE; FOODS; Fundamental and applied biological sciences. Psychology; HIGADO; HUILE D'OLIVE; HUILE DE POISSON; hydrocymethylglutaryl-CoA reductase; LABORATORY ANIMALS; LIVER; Male; marine fish oil; membrane fluidity; Membrane Fluidity - drug effects; Membrane Lipids - chemistry; Membrane Lipids - metabolism; Membrane Lipids - physiology; Membrane physicochemistry; Microsomes, Liver - drug effects; Microsomes, Liver - enzymology; Molecular biophysics; OLIVE OIL; OXIDOREDUCTASES; OXIDORREDUCTASAS; OXYDOREDUCTASE; Plant Oils - administration & dosage; PRODUIT ALIMENTAIRE; PROPIEDADES REOLOGICAS; PROPRIETE RHEOLOGIQUE; public health; RAT; RATA; RATS; Rats, Inbred Strains; RHEOLOGICAL PROPERTIES; TRANSFERASAS; TRANSFERASE; TRANSFERASES; UNSATURATED FATTY ACIDS; acyl-CoA; fatty acid composition; hydrocymethylglutaryl-CoA reductase; cholesterol; olive oil; desaturase; marine fish oil; membrane fluidity; Rat; Liver; Rodentia; Polyunsaturated fatty acid; Olive oil; Microsome; Fatty acids; Fish oil; Cholesterol; Membrane enzyme; Vertebrata; Mammalia; Diet; Plasma membrane
• ISSN: 0300-9084; 1638-6183
• DOI: 10.1016/0300-9084(92)90153-6

Male rats were fed diets containing olive or marine fish oils (10% w/w) with or without added cholesterol (1% w/w). After six weeks of feeding, the major fatty acid composition, fluidity, fatty acid desaturating and cholesterol biosynthesis/esterification related enzymes of liver microsomes were determined. Both olive oil and marine fish oil diets, without added cholesterol, enriched content of oleic and docosahexaenoic acids, respectively, of rat liver microsomes. The results were consistent with reduction in Δ 6 and Δ 5 desaturation on n-6 essential fatty acids and higher fluidity in the marine origin oil group. Inclusion of cholesterol into diets resulted in decreased membrane arachidonic acid content, with concomitant increase in linoleic acid content. Cholesterol feeding also decreased Δ 6 and Δ 5 desaturase activities, as well as membrane fluidity. Furthermore, the activity of acyl-CoA:cholesterol acyltransferase dexreased, whereas the activity of hydroxymethylglutaryl-CoA reductase increased, in liver microsomes from both cholesterol-fat groups.