The effect of simulated gastrointestinal digestion on shrimp Penaeus vannamei allergenicity

• Published in: Chinese journal of oceanology and limnology Vol. 27; no. 4; pp. 703 - 707
• Main Author: 郭永超 李振兴 林洪
• Format: Journal Article
• Language: English
• Published: Heidelberg SP Science Press 01.11.2009; Springer Nature B.V; Food Safety Laboratory,Ocean University of China,Qingdao 266003,China
• Subjects: Allergenicity; Allergic reactions; Allergies; Body fluids; Crustaceans; Decapoda; Diagnostic tests; Digestion; Digestive system; Earth and Environmental Science; Earth Sciences; ELISA; ELISA法; Enzyme-linked immunosorbent assay; Fluids; Gastroenterology; Gastrointestinal tract; Immunoblotting; Litopenaeus vannamei; Oceanography; Penaeus vannamei; Shrimps; Simulation; 南美白对虾; 模拟胃液; 消化时间; 胃肠道; 致敏性; 过敏原; 过敏性; digestion; Pen a 1; SIF; SGF; allergenicity
• ISSN: 0254-4059; 2096-5508; 1993-5005; 2523-3521
• DOI: 10.1007/s00343-009-9190-3

Pen a 1 is a major shrimp allergen that induces an allergy. The aim of this study is to assess the effect of simulated gastrointestinal fluids on the allergenicity of Pen a 1. Purified Pen a 1 from shrimp （Penaeus vannamei） was subjected to digestion in SGF （simulated gastric fluid） and SIF （simulated intestinal fluid） for a set time. The allergenicity of digestive Pen a 1 was analyzed by immunoblotting and Ci-ELISA, using pool sera from patients with shrimp specific IgE. The results showed that Pen a 1 exhibited a decrease in allergenicity with increasing digestion time in the SGF and SIF. However, Pen a 1 exhibited strong resistance to digestive fluids, and all yielded fragments （33 kD, 23 kD, and 14 kD） showed allergic activity. Therefore, anti-digestion may be an important factor for Pen a 1 to induce an allergy.