Up-regulation of carcinoembryonic antigen-related cell adhesion molecule 1 in gastrointestinal cancer and its clinical relevance

• Published in: Acta Biochimica et Biophysica Sinica Vol. 49; no. 8; pp. 737 - 743
• Main Authors: Zhou, Muqing, Jin, Zhiming, Liu, Yiwen, He, Yiqing, Du, Yan, Yang, Cuixia, Wang, Yingzhi, Hu, Jiajie, Cui, Lian, Gao, Feng, Cao, Manlin
• Format: Journal Article Web Resource
• Language: English
• Published: China Oxford University Press 01.08.2017
• Subjects: Adult; Aged; Aged, 80 and over; Antigens, CD - blood; Antigens, CD - genetics; Antigens, CD - metabolism; Cell Adhesion Molecules - blood; Cell Adhesion Molecules - genetics; Cell Adhesion Molecules - metabolism; Enzyme-Linked Immunosorbent Assay; Female; Gastrointestinal Neoplasms - blood; Gastrointestinal Neoplasms - genetics; Gastrointestinal Neoplasms - metabolism; Gene Expression Profiling - methods; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; Male; Middle Aged; Protein Isoforms - blood; Protein Isoforms - genetics; Protein Isoforms - metabolism; Reverse Transcriptase Polymerase Chain Reaction; Short Communication; Up-Regulation; 临床意义; 免疫组织化学方法; 反转录聚合酶链反应; 恶性肿瘤; 癌胚抗原; 细胞粘附分子; 胃肠道肿瘤; 酶联免疫吸附试验; CEACAM1; ROC curve; gastrointestinal neoplasms; enzyme-linked immunosorbent assay
• ISSN: 1672-9145; 1745-7270
• DOI: 10.1093/abbs/gmx060

Serum carcinoembryonic antigen-related cell adhesion molecule 1 （CEACAM1） is dysregulated in various malignant tumors and has been associated with tumor progression. However, the expres- sion and regulatory mechanisms of serum CEACAM1 in gastrointestinal cancer are still unclear. The expression ratio of the CEACAM1-L and CEACAMI-S isoforms has seldom been investigated in gastrointestinal cancer. In this study, we intended to explore the expression and diagnostic value of CEACAM1 in gastrointestinal cancer. Serum CEACAM1 levels were measured by enzyme- linked immunosorbent assay. The protein expression and distribution of CEACAM1 in tumors were examined by immunohistochemical staining. The expression patterns and ratio of CEACAM1-L/S were analyzed by reverse transcription-polymerase chain reaction. The results showed that serum CEACAM1 levels were significantly higher in cancer patients than in healthy controls. CEACAM1 was found in secreted forms within the neoplastic glands, and its expression was more intense at the tumor invasion front. The CEACAM1-L/S （L：S） ratios were up-regulated during tumorigenesis. Our data suggest that the serum level of CEACAM1 may be used to discriminate gastrointestinal cancer patients from health controls.