Non-optimal levels of dietary selenomethionine alter splenocyte response and modify oxidative stress markers in female mice

• Published in: Food and chemical toxicology Vol. 45; no. 7; pp. 1147 - 1153
• Main Authors: Vega, Libia, Rodríguez-Sosa, Miriam, García-Montalvo, Eliud A., Del Razo, Luz María, Elizondo, Guillermo
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.07.2007; New York, NY Elsevier Science
• Subjects: animal models; Animals; Antioxidants - administration & dosage; Antioxidants - toxicity; B-Lymphocytes - drug effects; B-Lymphocytes - pathology; Biological and medical sciences; biomarkers; Biomarkers - metabolism; cell proliferation; Cell Proliferation - drug effects; Chemical and industrial products toxicology. Toxic occupational diseases; Cytokine regulation; Diet; dietary minerals; Dose-Response Relationship, Drug; Female; females; Glutathione; Glutathione - metabolism; glutathione peroxidase; Glutathione Peroxidase - metabolism; immune response; Immune System - drug effects; Immune System - pathology; Immunomodulation; immunostimulants; interleukin-12; Interleukin-12 - secretion; interleukin-4; Interleukin-4 - secretion; lipid peroxidation; Lipid Peroxidation - drug effects; Liver - drug effects; Liver - metabolism; Medical sciences; Metals and various inorganic compounds; methionine; Mice; Mice, Inbred C57BL; Oxidative stress; Oxidative Stress - drug effects; Proliferative responses; redox reactions; selenium; Selenomethionine - administration & dosage; Selenomethionine - toxicity; Spleen - drug effects; Spleen - immunology; Spleen - pathology; Spleen - secretion; splenocytes; Toxicology; Weight Gain - drug effects; Oxidative stress; Cytokine regulation; Immunomodulation; Proliferative responses; Glutathione; Toxicity; Rodentia; Cytokine; Oral administration; Splenocyte; Vertebrata; Mammalia; Mouse; Animal; Female
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2006.12.021

Many studies evaluating the effects of selenium (Se) status on immunity utilize inorganic Se, although selenomethionine (Se-Met) has been suggested to be more bioavailable and less toxic. In the current study, we investigated the effects of dietary Se-Met on immune system function and cellular redox status in C57BL/6N female mice fed with low (0.02 ppm), sufficient (0.2 ppm, control group), or excess Se-Met (2 ppm) in the diet for 50 days. Low Se-Met intake reduced glutathione peroxidase (GPx) activity and glutathione concentration without modifying lipoperoxidation. While low Se-Met intake also reduced the number of B cells in the spleen, it increased mitogen-induced proliferation, IL-4 and IL-12 secretion when compared to the sufficient Se-Met intake group. In comparison to controls, excess Se-Met intake increased splenocyte proliferation and reduced B cell numbers, IL-4, and IL-12 secretion without affecting oxidative stress markers. These data suggest that Se-Met supplementation should be carefully evaluated as it many influence immune function.