Characterization of Plasmid-Mediated aphA-3 Kanamycin Resistance in Campylobacter jejuni
A total of 254 isolates of Campylobacter jejuni and three isolates of Campylobacter coli , isolated from Sweden, Canada, and Egypt, were screened for kanamycin resistance. Eight strains of C. jejuni contained large plasmids that carried the aphA-3 kanamycin-resistance marker. In six plasmids, the ap...
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Published in | Microbial drug resistance (Larchmont, N.Y.) Vol. 10; no. 2; pp. 98 - 105 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
Mary Ann Liebert, Inc
01.06.2004
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Subjects | |
Online Access | Get full text |
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Summary: | A total of 254 isolates of
Campylobacter jejuni
and three isolates of
Campylobacter coli
, isolated from Sweden, Canada, and Egypt, were screened for kanamycin resistance. Eight strains of
C. jejuni
contained large plasmids that carried the
aphA-3
kanamycin-resistance marker. In six plasmids, the
aphA-3
gene was located downstream of an apparent insertion sequence, designated IS
607
*, which showed a considerable similarity to IS
607
, characterized on the chromosome of some
Helicobacter pylori
strains. In contrast,
the other plasmids carried the
aphA-3
gene as a part of a resistance cluster. This included three resistance markers encoding 6′-adenylyltransferase (
aadE
), streptothricin acetyltransferase (
sat
), and 3′-aminoglycoside phosphotransferase type III (
aphA-3
). The genetic
organization of this resistance cluster suggests that it has been acquired by
C. jejuni
from a Gram-positive organism. The IS
607
* element was also observed in kanamycin-susceptible strains of
C. jejuni
on plasmids mediating tetracycline resistance. The kanamycin-resistance
phenotype transferred along with tetracycline resistance by conjugation from four representative
C. jejuni
strains to a recipient strain of
C. jejuni
. The kanamycin-resistance determinant (
aphA-3
) was stably transferred from one of the four
C. jejuni
strains to a
recipient strain of
Escherichia coli
. However, the
C. jejuni
plasmid, which also carries the
tetO
gene, was not maintained in
E. coli
. Pulsed-field gel electrophoresis revealed the integration of approximately 50 kb of the plasmid into the chromosome of the
E.
coli
recipient. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 1076-6294 1931-8448 |
DOI: | 10.1089/1076629041310127 |