Deletion Analysis of the Subunit Genes of V-Type Na⁺-ATPase from Enterococcus hirae
The V₁Vo-ATPase from Enterococcus hirae catalyzes ATP hydrolysis coupled with sodium translocation. Mutants with deletions of each of 10 subunits (NtpA, B, C, D, E, F, G, H, I, and K) were constructed by insertion of a chloramphenicol acetyltransferase gene into the corresponding subunit gene in the...
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Published in | Journal of biochemistry (Tokyo) Vol. 139; no. 6; pp. 1045 - 1052 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
England
Oxford University Press
01.06.2006
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Subjects | |
Online Access | Get full text |
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Summary: | The V₁Vo-ATPase from Enterococcus hirae catalyzes ATP hydrolysis coupled with sodium translocation. Mutants with deletions of each of 10 subunits (NtpA, B, C, D, E, F, G, H, I, and K) were constructed by insertion of a chloramphenicol acetyltransferase gene into the corresponding subunit gene in the genome. Measurements of cell growth rates, ²²Na⁺ efflux activities, and ATP hydrolysis activities of the membranes of the deletion mutants indicated that V-ATPase requires nine of the subunits, the exception being the NtpH subunit. The results of Western blotting and V₁-ATPase dissociation analysis suggested that the A, B, C, D, E, F, and G subunits constitute the V₁ moiety, whereas the V₀ moiety comprises the I and K subunits. |
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Bibliography: | To whom correspondence should be addressed. Phone: +81-4-7124-1501 (Ext. 4405), Fax: +81-4-7125-1841, E-mail, iyamato@rs.noda.tus.ac.jp istex:84FCB0FE401E3D2B3B0DFB0DD38E496D57198220 ark:/67375/HXZ-Q88RX0FC-W local:mvj108 |
ISSN: | 0021-924X 1756-2651 |
DOI: | 10.1093/jb/mvj108 |