Epigenetic silencing of microRNA-335 contributes to nasopharyngeal carcinoma metastasis

MircoRNA-335 (miR-335), a member of mircoRNAs (miRNAs) family, has been found to be correlated with tumor invasion and metastasis. In this study, we aimed to detect the effect of miR-335 methylation on metastasis of nasopharyngeal carcinoma. RT-PCR and methylation-specific PCR were applied to detect...

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Published inAmerican journal of otolaryngology Vol. 41; no. 1; p. 102302
Main Authors Yang, Ju-hong, Lin, Lie-Kun, Zhang, Song
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.01.2020
Elsevier Limited
Subjects
Breast cancer
Cancer
Deoxyribonucleic acid
DNA
DNA methylation
Epigenesis, Genetic
Epigenetics
Experiments
Gene expression
Gene Silencing
Head & neck cancer
Humans
Lymph nodes
Lymphatic system
Metastases
Metastasis
Methylation
MicroRNAs
MicroRNAs - genetics
miR-335
miRNA
Nasopharyngeal carcinoma
Nasopharyngeal Carcinoma - genetics
Nasopharyngeal Carcinoma - pathology
Neoplasm Metastasis - genetics
Neoplasm Staging
Patients
Polymerase chain reaction
Ribonucleic acid
RNA
Throat cancer
Tissues
Tumors
China
Nasopharyngeal carcinoma
miR-335
Metastasis
Methylation
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Summary:MircoRNA-335 (miR-335), a member of mircoRNAs (miRNAs) family, has been found to be correlated with tumor invasion and metastasis. In this study, we aimed to detect the effect of miR-335 methylation on metastasis of nasopharyngeal carcinoma. RT-PCR and methylation-specific PCR were applied to detect the expression levels of miR-335 and miR-335 methylation in nasopharyngeal carcinoma tissues. The levels of miR-335 expression were significantly lower in nasopharyngeal cancer tissues with promoter methylation, compared to those with promoter unmethylation. The levels of miR-335 gene promoter methylation were higher in 14 (46.7%) out of 30 nasopharyngeal cancer tissues. Furthermore, the patients with cervical lymph node metastasis had higher methylation rate in miR-335 promoter (66.7% versus 16.7%) than those without cervical lymph node metastasis. Gene methylation contributes the expression of miR-335 in nasopharyngeal carcinoma. The expression of miR-335 methylation is correlated with the metastasis of nasopharyngeal carcinoma.
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ISSN:0196-0709
1532-818X
DOI:10.1016/j.amjoto.2019.102302