Development of monoclonal antibodies against the abnormal prion protein isoform (PrP∨res) associated with chronic wasting disease (CWD)

• Published in: Journal of veterinary science (Suwŏn-si, Korea) Vol. 13; no. 4; pp. 429 - 432
• Main Authors: Jeong, H.J., Konkuk University, Seoul, Republic of Korea, Lee, N.H., Konkuk University, Seoul, Republic of Korea, Lee, J.B., Konkuk University, Seoul, Republic of Korea, Park, S.Y., Konkuk University, Seoul, Republic of Korea, Song, C.S., Konkuk University, Seoul, Republic of Korea, Seo, K.H., Konkuk University, Seoul, Republic of Korea, Kim, D.W., National Institute of Animal Science, RDA, Cheonan, Republic of Korea, Kim, Y.S., Ilsong Institute of Life Science, Hallym University, Anyang, Republic of Korea, Choi, I.S., Konkuk University, Seoul, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Korea (South) 대한수의학회 01.12.2012; The Korean Society of Veterinary Science
• Subjects: ALCE; Animals; Antibodies, Monoclonal - genetics; Blotting, Western - veterinary; CWD; Deer - immunology; Deer - metabolism; DNA Primers - genetics; ELAN; Electrophoresis, Polyacrylamide Gel - veterinary; ELKS; Endopeptidase K; Mice; Mice, Knockout; monoclonal antibody; Polymerase Chain Reaction - veterinary; PRION; PRIONES; PRIONS; Prions - genetics; Prions - immunology; Protein Isoforms - genetics; Protein Isoforms - immunology; rPres; Short Communication; Wasting Disease, Chronic - diagnosis; Wasting Disease, Chronic - immunology; 수의학; CWD; res< sup> elk; prion; monoclonal antibody; PrP&lt
• ISSN: 1229-845X; 1976-555X
• DOI: 10.4142/jvs.2012.13.4.429

Monoclonal antibodies (mAbs) specific for the abnormal prion protein isoform (PrP∨res) are indispensable for diagnosing chronic wasting disease (CWD). In this study, eight mAbs were developed by immunizing PrP knockout mice with recombinant elk PrP and an immunogenic PrP peptide. The reactivity of the mAbs to recombinant PrP and the PrP peptide was measured, and their isotypes were subsequently determined. Among them, four mAbs (B85-05, B85-08, B85-12, and B77-75) were shown by Western blotting to recognize proteinase K-treated brain homogenate derived from an elk suffering from CWD.