Sphingosine-1-phosphate induces early response gene expression in C6 glioma cells

• Published in: Brain research. Molecular brain research. Vol. 133; no. 2; pp. 325 - 328
• Main Authors: Segura, Bradley J., Zhang, Weizhen, Xiao, Liqun, Logsdon, Craig D., Mulholland, Michael W.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 18.02.2005; Elsevier
• Subjects: Animals; Biological and medical sciences; Blotting, Northern; C- fos; Cell Line, Tumor; Dose-Response Relationship, Drug; Drug Interactions; Early response gene; Estrenes - pharmacology; Fundamental and applied biological sciences. Psychology; Gene Expression - drug effects; Genes, fos - physiology; Glia; Glioma; Lysophosphatidic acid; Lysophospholipids - pharmacology; Mice; Pertussis Toxin - pharmacology; Phosphodiesterase Inhibitors - pharmacology; Pyrrolidinones - pharmacology; Receptors, Lysophosphatidic Acid - genetics; Receptors, Lysophosphatidic Acid - metabolism; Reverse Transcriptase Polymerase Chain Reaction - methods; RNA, Messenger - metabolism; Sphingosine - analogs & derivatives; Sphingosine - pharmacology; Sphingosine-1-phosphate; Sphingosylphosphorylcholine; Time Factors; Vertebrates: nervous system and sense organs; Early response gene; Lysophosphatidic acid; Sphingosine-1-phosphate; Sphingosylphosphorylcholine; Cellular and molecular biology; C- fos; Glia; Glial cell; Cell line; Neuroglia; C-fos; Gene expression; In vitro
• ISSN: 0169-328X; 1872-6941
• DOI: 10.1016/j.molbrainres.2004.10.016

Sphingosine-1-phosphate (S1P) caused dose-dependent and time-dependent increases in c- fos mRNA. Pretreatment with pertussis toxin (PTX; 100 ng/ml×24 h) reduced c- fos activation by S1P (100 μM—187±6% vs. 411±27%) and lysophosphatidic acid (LPA; 100 μM—90±34% vs. 188±41%), but not by sphingosylphosphorylcholine (SPC; 100 μM—390±47% vs. 420±44%). RT-PCR analysis and sequencing demonstrated the presence of previously unidentified LPA-responsive Endothelial Differentiation Gene (EDG) receptor mRNAs in C6 cells: EDG-2 and EDG-4.