Comparison of aneuploidy frequencies between in vitro matured and unstimulated cycles oocytes by metaphase comparative genomic hybridization (mCGH)

• Published in: Molecular biology reports Vol. 39; no. 5; pp. 6187 - 6191
• Main Authors: Yakut, Tahsin, Karkucak, Mutlu, Sher, Geoffrey, Keskintepe, Levent
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.05.2012; Springer Nature B.V
• Subjects: Adult; air; Aneuploidy; Animal Anatomy; Animal Biochemistry; Biomedical and Life Sciences; Biopsy; Carbon dioxide; Cell Differentiation; chromosome 11; Chromosome Aberrations; chromosomes; comparative genomic hybridization; Comparative Genomic Hybridization - methods; Embryos; Female; Genomics; Histology; human chorionic gonadotropin; Humans; Hybridization; In Vitro Oocyte Maturation Techniques - methods; Life Sciences; Metaphase; Molecular biology; Morphology; Oocytes; Oocytes - cytology; oxygen; patients; Polar Bodies - cytology; Polymerase chain reaction; polyploidy; Statistics; Comparative genomic hybridization; Aneuploidy; In vitro maturation
• ISSN: 0301-4851; 1573-4978; 1573-4978
• DOI: 10.1007/s11033-011-1436-4

A common observation after in vitro matured oocyte is that they yield poorer embryo quality compared to their in vivo counterparts. This study was designed to assess chromosomal status with metaphase comparative genomic hybridization after in vitro maturation (IVM) in unstimulated cycles and compare the results with those obtained after in vivo maturation. Patients without any obstetrical or gynecological pathology were admitted into the study. IVM oocytes were collected 36 h post hCG and matured in vitro at 37°C in 5% O 2 , 6% CO 2 , and 89% air for 36 h. All matured (metaphase II) oocytes were subject to polar body 1 (PB-1) biopsy and vitrified individually. PB-1 samples were transferred into 0.25 cc PCR tubes containing 2.5 μl of PBS. PB-1 samples from 12 IVM patients were studied. Twenty-six out of 63 PB-1 samples (41%) were determined as euploid and 37 samples (59%) were aneuploid, whereas these values were 42% euploid and 58% aneuploid in the control group (in vivo matured oocytes). No statistical differences were found between the IVM and the control groups for euploid–aneuploid samples ( P  = 0.900). More aneuploidy was observed on chromosomes 11, 13, 15, 21, and 22 after IVM. Results show a non-significant rate of abnormal PB-1 formation after IVM compared to in vivo maturation. More aneuploidy was observed in chromosomes 11, 13, 15, 21, and 22 in the IVM group.