Salidroside promotes the repair of spinal cord injury by inhibiting astrocyte polarization, promoting neural stem cell proliferation and neuronal differentiation

• Published in: Cell death discovery Vol. 10; no. 1; p. 224
• Main Authors: Qian, Dingfei, Dong, Yuan, Liu, Xiaole, Yu, Haichao, Song, Zelong, Jia, Chengqi, Zhang, Zhen, Cao, Shiqi, Hu, Fanqi, Zhang, Xuesong
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 09.05.2024; Springer Nature B.V; Nature Publishing Group
• Subjects: 631/378/2183/2182; 692/699/375/1824; Apoptosis; Astrocytes; Biochemistry; Biomedical and Life Sciences; c-Jun protein; Cell Biology; Cell Cycle Analysis; Cell differentiation; Cell migration; Cell proliferation; Hyperplasia; Immunofluorescence; Ischemia; JNK protein; Life Sciences; Myelination; Neural stem cells; Neuronal-glial interactions; Neurotoxicity; Phosphorylation; Polarization; Regeneration; Spinal cord injuries; Stat3 protein; Stem Cells; Transcription factors
• ISSN: 2058-7716; 2058-7716
• DOI: 10.1038/s41420-024-01989-2

Spinal cord injury (SCI) remains a formidable challenge, lacking effective treatments. Following SCI, neural stem cells (NSCs) migrate to SCI sites, offering a potential avenue for nerve regeneration, but the effectiveness of this intrinsic repair mechanism remains suboptimal. Salidroside has demonstrated pro-repair attributes in various pathological conditions, including arthritis and cerebral ischemia, and the ability to curtail early-stage inflammation following SCI. However, the specific role of salidroside in the late-stage repair processes of SCI remains less defined. In this investigation, we observed that continuous salidroside treatment in SCI mice improved motor function recovery. Immunofluorescence-staining corroborated salidroside’s capacity to stimulate nerve regeneration and remyelination, suppress glial scar hyperplasia, reduce the activation of neurotoxic A1 astrocytes, and facilitate NSCs migration towards the injured region. Mechanistically, in vitro experiments elucidated salidroside’s significant role in restraining astrocyte proliferation and A1 polarization. It was further established that A1 astrocytes hinder NSCs proliferation while inducing their differentiation into astrocytes. Salidroside effectively ameliorated this inhibition of NSCs proliferation through diminishing c-Jun N-terminal kinase (JNK) pathway phosphorylation and restored their differentiation into neurons by suppressing the signal transducer and activator of transcription 3 (STAT3) pathway. In summary, our findings suggest that salidroside holds promise as a therapeutic agent for traumatic SCI treatment.