Identification of immunodominant polypeptides common between Anaplasma centrale and Anaplasma marginale

• Published in: Veterinary immunology and immunopathology Vol. 29; no. 1; pp. 31 - 40
• Main Authors: Shkap, Varda, Pipano, Eugene, McGuire, Travis C., Palmer, Guy H.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.08.1991; Elsevier
• Subjects: ANAPLASMA; Anaplasma - immunology; Anaplasma marginale; Anaplasmosis - immunology; Anaplasmosis - microbiology; Animals; ANTIBODIES; Antibodies, Bacterial - immunology; ANTICORPS; ANTICUERPOS; Antigens, Bacterial - immunology; Bacterial Proteins - immunology; Bacteriology; Biological and medical sciences; BOVIN; CATTLE; Cross Reactions; DIAGNOSIS; DIAGNOSTIC; DIAGNOSTICO; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Fundamental and applied biological sciences. Psychology; GANADO BOVINO; Immunoblotting; Immunodominant Epitopes - analysis; Microbiology; Molecular Weight; PEPTIDE; PEPTIDES; PEPTIDOS; Rabbits; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; SDS; ELISA; Binding; Immunization; Antigenic determinant; Antibody; Induction; Cross protection; Anaplasmataceae; Anaplasma marginale; Immunodominance; Infection; Rickettsiales; Antigen; Cross reaction; Bacteriosis; Bacteria
• ISSN: 0165-2427; 1873-2534
• DOI: 10.1016/0165-2427(91)90050-M

High titered antibody from rabbits immunized with Anaplasma centrale or from cattle recovered from A. centrale infection bound predominantly to several 33–36 kDa polypeptides present in both A. centrale and the israel-NT isolate of Anaplasma marginale. High titered bovine antibody against the Israel-NT isolate of A. marginale also reacted predominantly with A. centrale polypeptides in this size range. The immunodominance of the 33–36 kDa polypeptides and their cross-reactivity indicate that these shared epitopes may be primarily responsible for the cross-prototective immunity between A. centrale and A. marginale.