gIII antibody responses in pigs following vaccination and challenge to Aujeszky's disease

• Published in: Veterinary microbiology Vol. 27; no. 2; pp. 197 - 201
• Main Authors: Motha, M.X.Julian, Ralston, Jacqueline C.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 1991; Elsevier Science
• Subjects: Animals; ANTIBODIES; Antibodies, Viral - biosynthesis; ANTICORPS; ANTICUERPOS; AUJESZKYS DISEASE; Biological and medical sciences; CERDO; ENFERMEDAD DE AUJESZKY; Enzyme-Linked Immunosorbent Assay; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Herpesvirus 1, Suid - immunology; HERPETOVIRIDAE; Immunobiology; MALADIE D'AUJESZKY; Neutralization Tests; PORCIN; Pseudorabies - immunology; Reagent Kits, Diagnostic; SWINE; Swine Diseases - immunology; VACCINATION; Vaccination - veterinary; VACUNACION; Viral Vaccines - immunology; Vertebrata; Mammalia; Immune response; Neutralization test; Antibody; Swine; Vaccination; Serum; Artiodactyla; ELISA assay; Ungulata; Humoral immunity
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/0378-1135(91)90011-4

Serological responses to a genetically engineered Aujeszky's disease “marker” vaccine (dl gIII + dl tk) were monitored using a blocking-ELISA (B-ELISA), a serum neutralisation test (SNT) and an indirect ELISA (I-ELISA). The B-ELISA is capable of differentiating pigs vaccinated with the above vaccine from natural infection. The SNT and the I-ELISA indicated that the pigs responded to vaccination and challenge. All three tests showed that the controls and the in-contact pigs always reacted negative for antibodies. The B-ELISA was able to detect pigs challenged with a field isolate 24 days post-challenge. These pigs remained positive until 110 days post-challenge when last tested. These findings indicate that the B-ELISA could be used successfully with this vaccine in a control eradication programme. This trial also shows that the vaccine virus did not spread to the in-contact pigs and also the vaccinated and challenged pigs did not transmit the disease to other susceptible pigs when they were introduced 14 days after challenge.