Sargahydroquinoic acid isolated from Sargassum serratifolium as inhibitor of cellular basophils activation and passive cutaneous anaphylaxis in mice

• Published in: International immunopharmacology Vol. 105; p. 108567
• Main Authors: Seong Choi, Kap, Shin, Tai-Sun, Chun, Jiyeon, Ahn, Ginnae, Jeong Han, Eui, Kim, Min-Jong, Kim, Jung-Beom, Kim, Sang-Hyun, Kho, Kang-Hee, Heon Kim, Dae, Shim, Sun-Yup
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2022; Elsevier BV
• Subjects: 12-O-Tetradecanoylphorbol-13-acetate; Acetic acid; Alkenes; Allergic reactions; Anaphylaxis; Anaphylaxis - metabolism; Animal models; Animals; Antioxidants; Basophilic leukemia; Basophils; Benzoquinones; Bone marrow; Bovine serum albumin; Calcimycin; Calcium; Calcium ionophores; Cell activation; Compound 48/80; Cytokines; Degranulation; Effector cells; Food allergies; Functional foods & nutraceuticals; Gene expression; Immunoglobulin E; Interleukin 1; Interleukin 4; Interleukin 6; Interleukin 8; Leukemia; Leukocytes (basophilic); Mast Cells; Mice; Mice, Inbred BALB C; Passive Cutaneous Anaphylaxis; Rats; Reactive oxygen species; Sargahydroquinoic acid; Sargassum; Sargassum serratifolium; Serum albumin; Tumor necrosis factor-TNF; Degranulation; Sargassum serratifolium; Passive cutaneous anaphylaxis; Sargahydroquinoic acid; Cytokines; Basophils
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2022.108567

•Sargahydroquinoic acid, SHQA was isolated from marine algae, Sargassum serratifolium.•SHQA inhibited calcium influx, ROS and cytokines production, MAPK and NF- k B activation, degranulation in PMACI-induced KU812F cells.•SHQA inhibited the cytokines production and b -hexosaminidase release in com 48/80-induced RBL-2H3 cells.•SHQA inhibited the degranulation in IgE-mediated BMCMCs and PCA in mice.•SHQA isolated from Sargassum serratifoliumcould be used as potential therapeutic functional food materials for protection of allergic disorders. Basophils and mast cells are characteristic effector cells in allergic reactions. Sargahydorquinoic acid (SHQA), a compound isolated from Sargassum serratifolium (marine alga), possesses various biochemical properties, including potent antioxidant activities. The objective of the present study was to investigate inhibitory effects of SHQA on the activation of human basophilic KU812F cells induced by phorbol myristate acetate and A23187 (PMACI), a calcium ionophore. Furthermore, we confirmed the inhibitory effects of SHQA on the activation of rat basophilic leukemia (RBL)–2H3 cells induced by compound 48/80 (com 48/80), bone marrow-derived mast cells (BMCMCs) induced by anti-dinitrophenyl(DNP)-immunoglobulin E (IgE)/DNP-bovine serum albumin (BSA), DNP/IgE and on the reaction of passive cutaneous anaphylaxis (PCA) mediated by IgE. SHQA reduced PMACI-induced intracellular reactive oxygen species (ROS) and calcium levels. Western blot analysis revealed that SHQA downregulated the activation of ERK, p38, and NF-κB in a dose-dependent manner. Moreover, SHQA suppressed the production and gene expression of various cytokines, including interleukin (IL)-1 β, IL-4, IL-6, and IL-8 in PMACI-induced KU812F cells and IL-4 and tumor necrosis factor (TNF)- α in com 48/80-induced RBL-2H3 cells. It also determined the inhibition of PMACI, com 48/80- and IgE/DNP-induced degranulation by reducing the release of β -hexosaminidase. Furthermore, it attenuated the IgE/DNP-induced PCA reaction in the ears of BALB/c mice. These results suggest that SHQA isolated from S. serratifolium is a potential therapeutic functional food material for inhibiting effector cell activation in allergic reactions and anaphylaxis in animal model.