Low-Temperature Plasma-Activated Medium Inhibits the Migration of Non-Small Cell Lung Cancer Cells via the Wnt/ β -Catenin Pathway

• Published in: Biomolecules (Basel, Switzerland) Vol. 13; no. 7; p. 1073
• Main Authors: Zhang, Yan, Yan, Zhuna, Wu, Hui, Yang, Xiao, Yang, Ke, Song, Wencheng
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 04.07.2023; MDPI
• Subjects: Apoptosis; Cancer therapies; Cell adhesion & migration; Cell culture; Cell division; Cell migration; Cell viability; Chemotherapy; Experiments; Low temperature; Lung cancer; Medical research; Melanoma; Membrane potential; Metastasis; migration; Molecular modelling; Non-small cell lung carcinoma; NSCLC; Plasma; plasma-activated medium; Radiation therapy; RONS; Signal transduction; Skin cancer; Small cell lung carcinoma; Wnt protein; Wnt/β-catenin; Wound healing; β-Catenin; United States > US; China; migration; plasma-activated medium; NSCLC; RONS; Wnt/β-catenin
• ISSN: 2218-273X; 2218-273X
• DOI: 10.3390/biom13071073

This study explored the molecular mechanism of the plasma activation medium (PAM) inhibiting the migration ability of NSCLC (non-small cell lung cancer) cells. The effect of PAM incubation on the cell viability of NSCLC was detected through a cell viability experiment. Transwell cells and microfluidic chips were used to investigate the effects of PAM on the migration capacity of NSCLC cells, and the latter was used for the first time to observe the changes in the migration capacity of cancer cells treated with PAM. Moreover, the molecular mechanisms of PAM affecting the migration ability of NSCLC cells were investigated through intracellular and extracellular ROS detection, mitochondrial membrane potential, and Western blot experiments. The results showed that after long-term treatment with PAM, the high level of ROS produced by PAM reduced the level of the mitochondrial membrane potential of cells and blocked the cell division cycle in the G2/M phase. At the same time, the EMT process was reversed by inhibiting the Wnt/ -catenin signaling pathway. These results suggested that the high ROS levels generated by the PAM treatment reversed the EMT process by inhibiting the WNT/ -catenin pathway in NSCLC cells and thus inhibited the migration of NSCLC cells. Therefore, these results provide good theoretical support for the clinical treatment of NSCLC with PAM.