Neuropathological studies on Plasmodium yoelii nigeriensis-induced malaria in mice

• Published in: Journal of comparative pathology Vol. 110; no. 3; pp. 313 - 317
• Main Authors: Sharma, M.R., Sharma, M.C., Tripathi, L.M., Pandey, V.C., Maitra, S.C.
• Format: Journal Article
• Language: English
• Published: London Elsevier Ltd 01.04.1994; Elsevier
• Subjects: Animals; Biological and medical sciences; Brain - blood supply; Brain - pathology; Endothelium, Vascular - pathology; Experimental protozoal diseases and models; Infectious diseases; Malaria, Cerebral - pathology; Medical sciences; Mice; Microscopy, Electron; Parasitic diseases; Plasmodium yoelii; Protozoal diseases; Protozoa; Protozoal disease; Nervous system diseases; Malaria; Pathogenesis; Rodentia; Red blood cell; Veterinary medicine; Electron microscopy; Infection; Vertebrata; Experimental disease; Mammalia; Histopathology; Mouse; Blood vessel; Plasmodium yoelii nigeriensis; Cerebral edema; Sporozoa; Brain (vertebrata)
• ISSN: 0021-9975; 1532-3129
• DOI: 10.1016/S0021-9975(08)80285-X

Malaria infection in mice was produced by intraperitoneal inoculation of 10 6 erythrocytes parasitized with Plasmodium yoelii nigeriensis, a virulent strain of murine malaria. About one week after infection parasitaemia ranged between 60 and 80%, and 100% mortality was observed. Infected animals were killed 6 days after infection to allow the examination of brain tissue. Electron microscopical observations revealed marked damage to cerebral vascular vessel walls with separation of muscular layers, media and adventitia. The endothelial cell layer was discontinuous in places. Activated fibroblast cells producing collagen fibres were seen around the necrotic region of cerebral vasculature. Some parasitized erythrocytes were also seen attached to the endothelial cell lining. Cerebral oedema was prominent around the blood vessels.