Culture media for the isolation and enumeration of pathogenic Vibrio species in foods and environmental samples

• Published in: International Journal of Food Microbiology [INT. J. FOOD MICROBIOL.]. Vol. 26, no. 1. 1995 Vol. 26; no. 1; pp. 77 - 91
• Main Authors: Donovan, Terence J., van Netten, Peter
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 01.06.1995; Elsevier
• Subjects: ALIMENTOS; ANALISIS MICROBIOLOGICO; ANALYSE MICROBIOLOGIQUE; Biological and medical sciences; Colony Count, Microbial; CULTURE MEDIA; Culture Media - chemistry; ENFERMEDADES TRANSM POR ALIMENTOS; Food industries; Food Microbiology; Food surveillance; FOODBORNE DISEASES; FOODS; Fundamental and applied biological sciences. Psychology; MALADIE TRANSMISSIBLE PAR ALIMENT; MEDIO DE CULTIVO; MICROBIOLOGICAL ANALYSIS; MILIEU DE CULTURE; PRODUIT ALIMENTAIRE; Species Specificity; VIBRIO; Vibrio - classification; Vibrio - isolation & purification; Vibrios; Food surveillance; Culture media; Vibrios; Foodborne diseases; Culture medium; Selective medium; Microbiological testing; Foodstuff; Environmental control; Medium enrichment; Pathogenic; Quality control; Analytical method; Bacteria; Vibrionaceae; Biological contamination; Vibrio
• ISSN: 0168-1605; 1879-3460
• DOI: 10.1016/0168-1605(95)00015-C

The genus Vibrio now includes a large number of species. Clear evidence is only available for the aetiological role of V. cholerae, V. vulnificus and V. parahaetnolyticus in foodborne diseases. Until recently, V. cholerae serogroup 0:1 was accepted as the cause of epidemic cholera. However, the designation of outbreaks of diarrhoeal diseases caused by V. cholerae 0:139 as clinical cholera has lead to renewed interest in Non 0:1 serogroups of V. cholerae. A wide range of enrichment and selective media for the isolation of vibrios has been developed. These media are reviewed with respect to their ability to recover and differentiate the target vibrios. Alkaline peptone water (APW) remains the recommended enrichment medium for vibrios in parallel with either salt polymyxin broth (SPB) or glucose teepol (or sodium dodecylsulphate) salt broth (GTSB) when tests for V. parahaemolyticus are required. Thiosulpbate citrate bile salt agar (TCBS) in parallel with polymyxin mannose tellurite (PMT) or sodium dodecylsulphate polymyxin sucrose agar (SPS) are the recommended selective plating media.