Loss of thioredoxin reductase function in a mouse stroke model disclosed by a two-photon fluorescent probe

• Published in: Chemical communications (Cambridge, England) Vol. 56; no. 9; pp. 1475 - 1478
• Main Authors: Zhao, Jintao, Qu, Yuan, Gao, Hao, Zhong, Miao, Li, Xinming, Zhang, Fang, Chen, Yaxiong, Gan, Lu, Hu, Guodong, Zhang, Hong, Zhang, Shengxiang, Fang, Jianguo
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 12.11.2020
• Subjects: Animals; brain; Disease Models, Animal; fluorescent dyes; Fluorescent Dyes - chemistry; Fluorescent Dyes - metabolism; Fluorescent indicators; HeLa Cells; Hep G2 Cells; Homeostasis; Humans; Mice; Molecular Structure; oxidoreductases; Phosphines - chemistry; Phosphines - metabolism; Photons; Reductases; stroke; Stroke - metabolism; Thioredoxin-Disulfide Reductase - metabolism; thioredoxins; Zebrafish
• ISSN: 1359-7345; 1364-548X; 1364-548X
• DOI: 10.1039/d0cc05900e

Thioredoxin reductase (TrxR) enzymes are critical in regulating redox homeostasis in cells. We report the first two-photon fluorescent probe of mammalian TrxR (TP-TRFS). TP-TRFS retains high specificity in recognizing TrxR. More importantly, the two-photon absorbing character of TP-TRFS enables it to be used in vivo . With the aid of TP-TRFS, a remarkable decline of the TrxR function was observed in the brain of a mouse model of stroke for the first time, providing a mechanistic link of TrxR dysfunction with stroke. The first two-photon fluorescent probe (TP-TRFS) is reported, and it was successfully used in vivo .