Expression of γ-synuclein in colorectal cancer tissues and its role on colorectal cancer cell line HCT116

• Published in: World journal of gastroenterology : WJG Vol. 15; no. 40; pp. 5035 - 5043
• Main Authors: Ye, Qing, Feng, Bo, Peng, Yuan-Fei, Chen, Xue-Hua, Cai, Qu, Yu, Bei-Qin, Li, Liang-Hui, Qiu, Ming-Yuan, Liu, Bing-Ya, Zheng, Min-Hua
• Format: Journal Article
• Language: English
• Published: United States Shanghai Institute of Digestive Surgery, Shanghai 200025, China%Shanghai Institute of Digestive Surgery, Shanghai 200025, China 28.10.2009; Shanghai Minimally Invasive Surgery Center, Shanghai 200025,China; Department of General Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine; The WJG Press and Baishideng
• Subjects: Cell Line, Tumor; Cell Movement; Cell Proliferation; Colorectal Neoplasms - metabolism; Disease Progression; gamma-Synuclein - biosynthesis; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry - methods; Neoplasm Invasiveness; Original; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; RNA, Small Interfering - metabolism; Time Factors; 核蛋白; 癌组织; 癌细胞; Cell proliferation; Expression; Migration; Colorectal cancer; γ-synuclein; Colony formation; Invasion
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.15.5035

AIM： To investigate the expression pattern of γ-synuclein in colorectal cancer （CRC） tissues, and to study the effects of γ-synuclein on CRC cell line HCT116 biological features in vitro.METHODS： The expression pattern of γ-synuclein was determined in 54 CRC tissues and 30 tumor-matched nonneoplastic adjacent tissues （NNAT） 5 cm away from the tumor via real-time quantitative reverse transcription PCR （RT-PCR） and immunohistochemistry. The relationship between γ-synuclein protein expression and clinicopathological factors of CRC tissues was analyzed. Three small interfering RNA （siRNA） targeting γ-synuclein mRNA plasmids were constructed and transfected into the CRC cell line HCT116. The stable cell lines were selected with G-418 for 28 d, and the biological features of these cells were examined by cell growth curve, soft agar assay, and cell migration and invasion assays in vitro. RESULTS： The expression of γ-synuclein mRNA and protein was much higher in CRC tissue samples than in NNAT samples （P = 0.02, P = 0.036）. There was a significant correlation between the γ-synuclein protein expression and clinical stage and lymph node involvement of CRC （P = 0.02, P = 0.033）. In functional analysis we found that down-regulation of γ-synuclein expression in HCT116 cells could inhibit the growth, colony formation rate, and migration and invasion ability of HCT116 cells.CONCLUSION： Increased expression of γ-synuclein in CRC tissues and the biological effects of reduced γ-synuclein expression on HCT116 cells suggest that γ-synuclein may play a positive role in the progression of CRC.