The dual roles of Ganoderma antioxidants on urothelial cell DNA under carcinogenic attack

• Published in: Journal of ethnopharmacology Vol. 118; no. 2; pp. 324 - 330
• Main Authors: Yuen, J.W.M., Gohel, M.D.I.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 23.07.2008; Amsterdam; New York: Elsevier; Elsevier
• Subjects: Aminobiphenyl Compounds - toxicity; anticarcinogenic activity; antioxidant activity; Antioxidants; Antioxidants - administration & dosage; Antioxidants - isolation & purification; Antioxidants - pharmacology; apoptosis; Biological and medical sciences; Bladder cancer; bladder diseases; Cell Line; chemoprevention; cultured cells; cytotoxicity; Deoxyguanosine - analogs & derivatives; Deoxyguanosine - metabolism; DNA damage; DNA Damage - drug effects; Dose-Response Relationship, Drug; Drugs, Chinese Herbal - administration & dosage; Drugs, Chinese Herbal - isolation & purification; Drugs, Chinese Herbal - pharmacology; Ferric Compounds - chemistry; Ganoderma; Ganoderma lucidum; General pharmacology; herbal medicines; human cell lines; Humans; Hydrogen Peroxide - metabolism; Medical sciences; medicinal properties; neoplasms; Oxidative DNA damage; oxidative stress; Oxidative Stress - drug effects; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; protective effect; Reishi - chemistry; Urothelium - cytology; Urothelium - drug effects; Urothelium - metabolism; Yin–Yang; Antioxidants; Ganoderma lucidum; Bladder cancer; Yin–Yang; Oxidative DNA damage; Pharmacognosy; Malignant tumor; Antioxidant; In vitro; Basidiomycota; Medicinal plant; Fungi; Carcinogen; Urinary system; Urinary bladder; DNA; Wood destroying fungi; Plant origin; Oxidative DNA damage,Antioxidants,Ganoderma lucidum,Bladder cancer,Yin-Yang; Lesion; Cancer
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2008.05.003

Ganoderma lucidum possesses significant antioxidant and anti-cancer properties. The effects of Lingzhi antioxidants on oxidative DNA damage and oxidative stress were investigated, in order to understand their roles in bladder chemoprevention. Antioxidant-enhanced extracts were tested, in terms of ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH·) inhibition, hydrogen peroxide (H 2O 2) and 8-hydroxy-2′-deoxyguanosine (8-OHdG) concentrations, and cytotoxicity, using an in vitro pre-cancerous human uroepithelial cell (HUC-PC) tumorigenic model. Water-soluble extract (GLw) possesses relatively higher antioxidant capacities than the water-insoluble counterpart (GLe); however, under the challenge of carcinogenic 4-aminobiphenyl (ABP), GLw reduced the 8-OHdG concentration in HUC-PC culture, while GLe induced the formation of H 2O 2 and 8-OHdG in a dose-dependent manner. The modern theory of antioxidant–oxidant balance seems to obey the theory of Yin–Yang in traditional Chinese medicine (TCM). Water-soluble and water-insoluble components of Ganoderma lucidum exhibited dual roles in oxidative DNA damage. Oxidative DNA damage may be an underlying mechanism of Lingzhi-induced apoptosis in bladder chemoprevention.