Early detection of Trichinella spiralis infection by the polymerase chain reaction in blood samples of experimentally infected mice

• Published in: Parasite (Paris) Vol. 8; no. Supplément au n° 2; pp. S229 - S231
• Main Authors: Caballero-Garcia, M.L., Jimenez-Cardoso, E.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Paris EDP Sciences 01.06.2001; Princeps
• Subjects: Animals; Biological and medical sciences; Disease Models, Animal; DNA; DNA, Helminth - analysis; Electrophoresis, Agar Gel; Experimental helminthic diseases. Models; Female; Helminthic diseases; Infectious diseases; Larva; Medical sciences; Mice; Mice, Inbred BALB C; Parasitic diseases; PCR; Polymerase Chain Reaction - methods; T. spiralis; Trichinella spiralis - genetics; Trichinella spiralis - isolation & purification; trichinellosis; Trichinellosis - diagnosis; Rodentia; Parasite; Parasitosis; Helminthiasis; Blood; Infection; Polymerase chain reaction; Vertebrata; Mammalia; Mouse; Pathogenic; Nematode disease; Helmintha; Nemathelminthia; Trichinella spiralis; Diagnosis; Invertebrata; Nematoda; Trichinosis
• ISSN: 1252-607X; 1776-1042
• DOI: 10.1051/parasite/200108s2229

Up to now, there is no useful method to diagnose trichinellosis at the early stages of infection. The aim of the present investigation was to know if the polymerase chain reaction (PCR), could detect DNA of migratory larvae in mice experimentally infected with T. spiralis. Thirty- three Balb/c female mice 4.6 weeks old, were infected with 300 larvae of T. spiralis /mouse. Blood samples of these animals were positive for T. spiralis at 3th and 17t h postinfection days. The control group was negative. PCR could detect from one to 200 larvae. Our results showed that it was possible to make early diagnosis of trichinellosis in blood of mice infected with T. spiralis.