Expression and influence of KATP in umbilical artery smooth muscle cells of patients with hypertensive disorders of pregnancy

• Published in: Scientific reports Vol. 14; no. 1; p. 7517
• Main Authors: Yin, Benlan, Yu, Xiaotong, Fu, Xiaodong, Liu, Xiyuan, Xiao, Jing, Yu, Linli, Nie, Yunying, Zhang, Yujiao
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 29.03.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 692/4019; 692/699; Adenosine Triphosphate - metabolism; ATP; Female; Humanities and Social Sciences; Humans; Hypertension; Hypertension, Pregnancy-Induced; Hypertensive disorders; KATP; KATP Channels - genetics; KATP Channels - metabolism; multidisciplinary; Myocytes, Smooth Muscle - metabolism; Potassium; Potassium channels (inwardly-rectifying); Pre-eclampsia; Pre-Eclampsia - genetics; Preeclampsia; Pregnancy; Pregnancy complications; Protein expression; Proteins; Science; Science (multidisciplinary); Smooth muscle; Statistical analysis; Sulfonylurea Receptors - metabolism; Umbilical Arteries - metabolism; Umbilical artery; Western blotting; Pregnancy; KATP; Umbilical artery; Hypertensive disorders
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-57885-3

The objective of this study is to investigate the expression and influence of adenosine triphosphate-sensitive potassium channel (KATP) in human umbilical arterial smooth muscle cells (HUASMCs) of patients with hypertensive disorders of pregnancy (HDP). Western blotting was used to detect the protein expression levels of KATP inwardly rectifying potassium channel (Kir)6.1 and sulphonylurea receptor (SUR)2B subunits in HUASMCs from patients with normal parturients (NP), gestational hypertension (GH), chronic hypertension (CH), preeclampsia (PE) and chronic hypertension with superimposed preeclampsia (CHSP), respectively. There was no significant difference in the protein expression of Kir6.1 subunit in NP group, GH group, CH group, PE group and CHSP group ( P  > 0.05). The protein expression of SUR2B subunit was gradually decreased in NP group, GH group, CH group, PE group and CHSP group, with statistically significant difference among the groups ( P  < 0.05). The altered expression level of KATP SUR2B subunit may be involved in the pathogenesis of HDP. The severity of HDP may be related to the degree of decrease of SUR2B subunit.