A capture antibody ELISA for detection of antibodies against bovine enterovirus

• Published in: Journal of virological methods Vol. 24; no. 1; pp. 223 - 226
• Main Authors: Zhang, A.Q., Smith, J.R., Burgess, G.W.
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 01.04.1989; Amsterdam Elsevier; New York, NY
• Subjects: Animals; ANTIBODIES; Antibodies, Viral - analysis; Antibody detection; ANTICORPS; ANTICUERPOS; Biological and medical sciences; BOVIN; Bovine enterovirus; Capture antibody ELISA; CATTLE; Chickens; ELISA; ENTEROVIRUS; Enterovirus - immunology; Enzyme-Linked Immunosorbent Assay; Evaluation Studies as Topic; Fundamental and applied biological sciences. Psychology; GANADO BOVINO; IgG F(ab') 2; Immunoglobulin Fab Fragments - immunology; Immunoglobulin G - immunology; Microbiology; Sensitivity and Specificity; Techniques used in virology; TEST ELISA; Virology; Antibody detection; Bovine enterovirus; Capture antibody ELISA; IgG F(ab') 2; Virus; Enterovirus; Picornaviridae; Antibody; ELISA assay
• ISSN: 0166-0934; 1879-0984
• DOI: 10.1016/0166-0934(89)90024-4

A capture antibody ELISA was developed for detecting antibodies against bovine enterovirus. Chicken IgG F(ab') 2 fragments against strain J2129 captured virus effectively. The captured virus was used to detect specific antibodies against the virus in bovine sera. The specificity of the ELISA is high. The factors affecting the specificity are discussed. The assay is more sensitive and economic than traditional serum neutralisation.