Amblyomma americanum (L.): Protein kinase C-independent fluid secretion by isolated salivary glands

• Published in: Experimental parasitology Vol. 74; no. 3; pp. 324 - 331
• Main Authors: McSwain, Janis L., Masaracchia, Ruthann A., Essenberg, R.C., Tucker, James S., Sauer, J.R.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.05.1992; Elsevier
• Subjects: ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; AMBLYOMMA AMERICANUM; Amblyomma americanum (L.); Amino Acid Sequence; Animals; Arachnida; Biochemistry. Physiology. Immunology; Biological and medical sciences; Calcium - pharmacology; Diglycerides - pharmacology; Diptera; Dopamine; Dopamine - pharmacology; ENZYME INHIBITORS; ENZYMIC ACTIVITY; Fundamental and applied biological sciences. Psychology; GLANDE SALIVAIRE; GLANDULAS SALIVALES; INHIBIDORES DE ENZIMAS; INHIBITEUR D'ENZYME; Invertebrates; Ixodidae; Molecular Sequence Data; Phosphatidylserines - pharmacology; Phosphoproteins - metabolism; Physiology. Development; PROTEIN KINASE; Protein kinase C; Protein Kinase C - metabolism; PROTEINA QUINASA; PROTEINE KINASE; SALIVARY GLANDS; Salivary Glands - enzymology; Salivary Glands - metabolism; SECRECION; SECRETION; Tetradecanoylphorbol Acetate - pharmacology; Tick, parasitic; Ticks - drug effects; Ticks - enzymology; Ticks - metabolism; 1-Oleoyl-2-acetyl-sn-glycerol; Protein kinase C inhibitor; Protein kinase C; Dopamine; Secretion; Phenylmethylsulfonyl fluoride; 3-( N-Morpholino)propanesulfonic acid; Fast protein liquid chromatography; Myosin light chain; Ethylenediaminetetraacetic acid; Tris(hydroxymethyl)aminomethane-hydrochloride; Tick, parasitic; Ethylene glycol bis(γ-aminoethyl ether) N,N,N′,N′- tetraacetic acid; Adenosine 5′-triphosphate; Sodium dodecyl sulfate-polyacrylamide gel electrophoresis; 12- O-Tetradecanoyl-phorbol 13-acetate; Adenosine 3′:5′-cyclic mono-phosphate; Amblyomma americanum (L.); Piperazine N, N′-bis(2-ethanesulfonic acid; Parasitiformes; Radiolabelling; Enzyme; Salivary gland; Stimulation; Chromatography; Arachnida; Acari; Enzymatic activity; Ixodida; Arthropoda; Amblyomma americanum; Electrophoresis; Ectoparasite; Inhibition; Invertebrata; Ixodidae
• ISSN: 0014-4894; 1090-2449
• DOI: 10.1016/0014-4894(92)90156-5

Protein kinase C activity was partially purified from tick salivary glands by fast protein liquid chromatography anion-exchange chromatography. Enzyme activity was stimulated by Ca 2+, phosphatidylserine, and diacylglycerol with the highest activity observed in the presence of all three modulators. Enzyme activity was inhibited by a synthetic pseudosubstrate peptide with an amino acid sequence resembling the protein kinase C substrate phosphorylation site. The protein kinase C activator, 1-oleoyl-2-acetyl-sn-glycerol (OAG), when added to whole in vitro salivary glands previously prelabeled with 32P, stimulated the phosphorylation of salivary gland proteins. Activators of protein kinase C (phorbol ester or OAG) did not stimulate fluid secretion by isolated tick salivary glands. OAG and phorbol ester had only minimal affects on the ability of dopamine to stimulate secretion by isolated salivary glands and dopamine's ability to increase salivary gland cyclic AMP.