A seroepidemiological survey for orthopox virus in the red fox ( Vulpes vulpes)

• Published in: Veterinary microbiology Vol. 43; no. 2; pp. 251 - 259
• Main Authors: Henning, Klaus, Czerny, Claus-Peter, Meyer, Hermann, Müller, Thomas, Kramer, Matthias
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.02.1995; Elsevier Science
• Subjects: Animal viral diseases; Animals; Antibodies, Viral - blood; Antibody Specificity; Biological and medical sciences; DISEASE SURVEYS; ENCUESTAS SANITARIAS; ENQUETE PATHOLOGIQUE; Enzyme-Linked Immunosorbent Assay - veterinary; Epidemiology; Female; FOXES; Foxes - blood; Foxes - immunology; Foxes - virology; Fundamental and applied biological sciences. Psychology; Germany - epidemiology; Infectious diseases; Male; Medical sciences; Microbiology; Orthopox virus; ORTHOPOXVIRUS; Orthopoxvirus - immunology; Poxviridae Infections - epidemiology; Poxviridae Infections - immunology; Poxviridae Infections - veterinary; Poxviridae Infections - virology; Prevalence; Red fox; RENARD; Seroepidemiologic Studies; Seroepidemiology; Viral diseases; Virology; Vulpes vulpes; ZORRO; Europe; Germany; Seroepidemiology; Red fox; Orthopox virus; Fissipedia; Carnivora; Chordopoxvirinae; Prevalence; Orthopoxvirus; Wild animal; Epidemiology; Fox; Infection; Virus; Vaccinia virus; Vertebrata; Mammalia; Poxviridae; Viral disease; Geographical variation; Vulpes vulpes
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/0378-1135(94)00097-G

703 blood samples from red foxes ( Vulpes vulpes) were investigated to determine the prevalence of antibody against an orthopox virus (vaccinia virus strain Elstree). A blocking-ELISA based on a neutralizing monoclonal antibody was used. In this assay 46 sera (6.5%) were positive with titers of 1:2 to 1:16. ELISA-results were confirmed by the plaque reduction test with 44 of the 46 sera reacting positively. The specificity of antibodies in 21 selected sera was also demonstrated by Western blot analysis.