Nosema chrysorrhoeae n. sp. (Microsporidia), isolated from browntail moth ( Euproctis chrysorrhoea L.) (Lepidoptera, Lymantriidae) in Bulgaria: Characterization and phylogenetic relationships

• Published in: Journal of invertebrate pathology Vol. 91; no. 2; pp. 105 - 114
• Main Authors: Hyliš, Miroslav, Pilarska, Daniela K., Oborník, Miroslav, Vávra, Jiří, Solter, Leellen F., Weiser, Jaroslav, Linde, Andreas, McManus, Michael L.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.02.2006; Elsevier
• Subjects: Animals; Biological and medical sciences; Bulgaria; developmental stages; DNA, Fungal - analysis; DNA, Fungal - genetics; DNA, Ribosomal - analysis; DNA, Ribosomal - genetics; Epithelial Cells - microbiology; Epithelial Cells - pathology; Epithelial Cells - ultrastructure; Euproctis chrysorrhoea; Fundamental and applied biological sciences. Psychology; Genes, rRNA - genetics; internal transcribed spacers; Lepidoptera; life cycle (organisms); Life Cycle Stages; Lymantria dispar; Lymantria monacha; Lymantriidae; Microsporidia; microsporidiosis; molecular systematics; Moths - microbiology; Muscles - microbiology; Muscles - pathology; Muscles - ultrastructure; new species; Nosema; Nosema - classification; Nosema - genetics; Nosema - growth & development; Nosema - isolation & purification; Nosema chrysorrhoeae; Nosema kovacevici; Nosema serbica; nucleotide sequences; Phylogeny; Protozoa; rDNA phylogeny; ribosomal DNA; Species Specificity; spores; Systematics. Geographical distribution. Morphology. Cytology; Taxonomy; Ultrastructure; Vairimorpha; Bulgaria; Europe; Nosema serbica; rDNA phylogeny; Ultrastructure; Taxonomy; Nosema chrysorrhoeae; Euproctis chrysorrhoea; Microsporidia; Nosema kovacevici; Protozoa; Entomopathogen; Lymantriidae; Insecta; Ribosomal DNA; Diagnosis (taxonomy); Parasite; Phylogeny; Cnidosporidia; Arthropoda; Morphology; Lepidoptera; New species; Invertebrata
• ISSN: 0022-2011; 1096-0805
• DOI: 10.1016/j.jip.2005.11.006

A new microsporidian parasite Nosema chrysorrhoeae n. sp., isolated in Bulgaria from the browntail moth ( Euproctis chrysorrhoea L.), is described. Its life cycle includes two sequential developmental cycles that are similar to the general developmental cycles of the Nosema-like microsporidia and are indistinguishable from those of two Nosema spp. from Lymantria dispar. The primary cycle takes place in the midgut tissues and produces binucleate primary spores. The secondary developmental cycle takes place exclusively in the silk glands and produces binucleate environmental spores. N. chrysorrhoeae is specific to the browntail moth. Phylogenetic analysis based on the ssu rRNA gene sequence places N. chrysorrhoeae in the Nosema/ Vairimorpha clade, with the microsporidia from lymantriid and hymenopteran hosts. Partial sequences of the lsu rRNA gene and ITS of related species Nosema kovacevici (Purrini K., Weiser J., 1975. Natürliche Feinde des Goldafters, Euproctis chrysorrhoea L., im Gebiet von Kosovo, FSR Jugoslawien. Anzeiger fuer Schädlingskunde, Pflanzen-Umweltschutz, 48, 11–12), Nosema serbica Weiser, 1963 and Nosema sp. from Lymantria monacha was obtained and compared with N. chrysorrhoeae. The molecular data indicate the necessity of future taxonomic reevaluation of the genera Nosema and Vairimorpha.