Quantitation of paraquat in biological samples by radioimmunoassay using a monoclonal antibody

• Published in: Fundamental and applied toxicology Vol. 19; no. 3; pp. 375 - 379
• Main Authors: Bowles, Mark R., Eyles, Darryl W., Hampson, Edith C.G.M., Pond, Susan M.
• Format: Journal Article
• Language: English
• Published: Boston, MA Elsevier Science (USA) 01.10.1992; San Diego, CA Academic Press; New York, NY
• Subjects: ANALISIS CUANTITATIVO; ANALYSE QUANTITATIVE; Animals; Antibodies, Monoclonal - immunology; Antibody Specificity; ANTICORPS MONOCLONAL; ANTICUERPOS MONOCLONALES; Biological and medical sciences; Bronchoalveolar Lavage Fluid - chemistry; Cells, Cultured; CHIEN; DIAGNOSTIC; DIAGNOSTICO; Dogs; ENVENENAMIENTO; Indicators and Reagents; Infusions, Intravenous; INTOXICATION; Iodine Radioisotopes; Medical sciences; Mice; Mice, Inbred BALB C; ORINA; PARACUAT; PARAQUAT; Paraquat - analysis; Paraquat - immunology; PERRO; Pesticides, fertilizers and other agrochemicals toxicology; PLASMA SANGUIN; PLASMA SANGUINEO; Radioimmunoassay; TECHNIQUE RADIOIMMUNOLOGIQUE; TECNICAS RADIOINMUNOLOGICAS; TOXICOLOGIA; TOXICOLOGIE; Toxicology; URINE; Urine; Fissipedia; Biological fluid; Carnivora; Intravenous administration; Pesticides; Monoclonal antibody; Herbicide; Blood plasma; Quaternary ammonium compound; Assay; Toxicokinetics; Vertebrata; Mammalia; Animal; Radioimmunoassay; Paraquat; Dog
• ISSN: 0272-0590; 1095-6832
• DOI: 10.1016/0272-0590(92)90176-I

We have developed a sensitive and specific radioimmunoassay for the quantitative determination of paraquat in plasma, urine, and bronchoalveolar lavage fluid using a monoclonal antibody. The synthesis of the iodinated paraquat tracer is novel and less complicated than a previously reported method. Regardless of the type of biological sample analyzed, the sensitivity of the assay was 0.46 ng/ml in a 200-μl aliquot. The results correlated well with those of another assay performed in a separate laboratory. Paraquat concentrations were determined in plasma and in urine of a dog over several days after the intravenous administration of 7.48 mg paraquat cation/kg and in bronchoalveolar lavage fluid obtained 34 hr after the 2-hr infusion was discontinued. Concentrations of paraquat measured ranged from 14.1 to 0.03 μg/ml in plasma and 2034 to 0.36 μg/ml in urine. Concentrations of paraquat in plasma obtained at various times after the ingestion of paraquat by three patients ranged from 51.0 to 0.010 μg/ml.